B7-H3/CD276 Antibody (RM335)
Novus Biologicals | Catalog # NBP2-77415
Recombinant Monoclonal Antibody
![Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody (RM335) [NBP2-77415]](https://resources.rndsystems.com/images/products/B7-H3-CD276-Antibody-RM335-Immunohistochemistry-NBP2-77415-img0001.jpg "Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody (RM335) [NBP2-77415]")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM335 expressed in HEK293
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Product Specifications
Immunogen
A peptide corresponding to the C-terminus of human B7-H3/CD276
Specificity
This antibody reacts to the cytoplasmic domain of human B7-H3/CD276.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for B7-H3/CD276 Antibody (RM335)
Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody (RM335) [NBP2-77415]
Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody (RM335) [NBP2-77415] - Staining of human colon cancer tissue section using NBP2-77415.
Western Blot: B7-H3/CD276 Antibody (RM335) [NBP2-77415] -
Western Blot: B7-H3/CD276 Antibody (RM335) [NBP2-77415] - Western Blot of LNCaP cell lysate using Clone RM335 at a 1:10000 dilution.Applications for B7-H3/CD276 Antibody (RM335)
Application
Recommended Usage
Immunohistochemistry
1:500 - 1:1000
Western Blot
1:5000-1:10000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50% Glycerol/PBS, 1% BSA
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: B7-H3
Long Name
B7 Homolog 3
Alternate Names
B7H3, CD276
Gene Symbol
CD276
Additional B7-H3 Products
Product Documents for B7-H3/CD276 Antibody (RM335)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for B7-H3/CD276 Antibody (RM335)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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