BACE-1 Antibody (3C1C3) - BSA Free

Novus Biologicals | Catalog # NBP2-37261

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3C1C3

Format

BSA Free
View all BACE-1 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human BACE-1 (AA: 112-324) expressed in E. Coli.

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:33776747).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

55.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BACE-1 Antibody (3C1C3) - BSA Free

Western Blot: BACE-1 Antibody (3C1C3)BSA Free [NBP2-37261]

Western Blot: BACE-1 Antibody (3C1C3)BSA Free [NBP2-37261]

Western Blot: BACE-1 Antibody (3C1C3) [NBP2-37261] - Analysis using BACE1 mouse mAb against Hela (1), SK-N-SH (2), HepG2 (3), C6 (4), PC-12 (5), PANC-1 (6), NIH/3T3 (7) cell lysate.
Immunocytochemistry/ Immunofluorescence: BACE-1 Antibody (3C1C3) - BSA Free [NBP2-37261]

Immunocytochemistry/ Immunofluorescence: BACE-1 Antibody (3C1C3) - BSA Free [NBP2-37261]

Immunocytochemistry/Immunofluorescence: BACE-1 Antibody (3C1C3) [NBP2-37261] - Analysis of MCF-7 cells using BACE1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Flow Cytometry: BACE-1 Antibody (3C1C3) - BSA Free [NBP2-37261]

Flow Cytometry: BACE-1 Antibody (3C1C3) - BSA Free [NBP2-37261]

Flow Cytometry: BACE-1 Antibody (3C1C3) [NBP2-37261] - Analysis of Hela cells using BACE1 mouse mAb (green) and negative control (red).
Western Blot: BACE-1 Antibody (3C1C3)BSA Free [NBP2-37261]

Western Blot: BACE-1 Antibody (3C1C3)BSA Free [NBP2-37261]

Western Blot: BACE-1 Antibody (3C1C3) [NBP2-37261] - Analysis using BACE1 mAb against human BACE1 (AA: 112-324) recombinant protein. (Expected MW is 49.9 kDa)
Western Blot: BACE-1 Antibody (3C1C3)BSA Free [NBP2-37261]

Western Blot: BACE-1 Antibody (3C1C3)BSA Free [NBP2-37261]

Western Blot: BACE-1 Antibody (3C1C3) [NBP2-37261] - Analysis using BACE1 mAb against HEK293 (1) and BACE1 (AA: 112-324)-hIgGFc transfected HEK293 (2) cell lysate.
ELISA: BACE-1 Antibody (3C1C3) - BSA Free [NBP2-37261]

ELISA: BACE-1 Antibody (3C1C3) - BSA Free [NBP2-37261]

ELISA: BACE-1 Antibody (3C1C3) [NBP2-37261] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
BACE-1 Antibody (3C1C3) - BSA Free

Western Blot: BACE-1 Antibody (3C1C3) - BSA Free [NBP2-37261] -

S@A-NPs treatment suppresses amyloidogenic cleavage of APP, while enhancing transcytosis of A beta out of the brains of APP/PS1 transgenic mice. (a) Schematic description of experimental timeline. APP/PS1 mice were intravenously injected with S@A-NPs at 6 months of age and subjected to analysis of A beta clearance at 7 months of age. SF555-conjugated A beta oligomers were stereotactically injected into the hippocampal DG. (b, c) SF555-conjugated A beta remnants in the brains were shown 48 h after injection. Area of SF555-conjugated A beta oligomers occupied in the hippocampal DG (c). (d, e) ELISA analysis of A beta 42 (d) and A beta 40 (e) levels in the plasma. (f) WB analysis of levels of full-length APP, BACE1 and alpha / beta -CTF in the cortex and hippocampus. GAPDH was detected as a loading control. Relative levels of full-length APP (f, g), BACE1 (f, h), beta -CTF (f, i), and alpha -CTF (f, j). Data are presented as mean +/- SEM. n = 3 mice/group (c); n = 7–9 mice/group (d); n = 6–11 mice/group (e); n = 3–8 mice/group (g-j). * p < 0.05; ** p < 0.01; *** p < 0.001. One-way ANOVA (c-e, g-j). Scale bars: 100 μm (b) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40830501), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for BACE-1 Antibody (3C1C3) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BACE-1

BACE1 is a protease responsible for the proteolytic processing of the amyloid precursor protein (APP). Amyloid beta peptide is the major constituent of amyloid plaques in the brains of individuals afflicted with Alzheimers disease. This peptide is generated from the beta-amyloid precursor protein (beta APP) in a two-step process. The first step involves cleavage of the extracellular, amino-terminal domain of beta APP. Protein cleavage is performed by an aspartyl protease termed beta-secretase (BACE), of which there are two isoforms, BACE1 and 2. This enzyme is synthesized as a propeptide that must be modified to the mature and active form by the prohormone convertase furin. Beta APP cleavage by the mature form of BACE results in the cellular secretion of a segment of beta APP and a membrane-bound remnant. This remnant is then processed by another protease termed gamma-secretase. Gamma-secretase cleaves an intra-membrane site in the carboxyl-terminal domain of beta APP, thus generating the amyloid beta peptide.

Long Name

beta-site Ayloid Precursor Protein Cleaving Enzyme 1

Alternate Names

BACE1

Gene Symbol

BACE1

Additional BACE-1 Products

Product Documents for BACE-1 Antibody (3C1C3) - BSA Free

Certificate of Analysis

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Product Specific Notices for BACE-1 Antibody (3C1C3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Aspartic Proteases and Regulators

Citations for BACE-1 Antibody (3C1C3) - BSA Free

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Protocols

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