Rat Bad ELISA Kit (Chemiluminescence)

Name: Rat Bad ELISA Kit (Chemiluminescence)
Brand: Novus Biologicals
SKU: NBP2-69921
Price: 809 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-69921

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Product Documents

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Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

7.50 pg/mL

Assay Range

12.50-800 pg/mL

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Product Specifications

Assay Type

Sandwich-CLIA

Kit Type

ELISA Kit (Chemiluminescence)

Species

Rat

Specificity

This kit recognizes Rat BAD in samples. No significant cross-reactivity or interference between Rat BAD and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 10.68%

Inter-Assay Precision (Precision between assays) CV% 10.4%

Recovery for Rat Bad ELISA Kit (Chemiluminescence)

Recovery

95-114%

Linearity

Scientific Data Images for Rat Bad ELISA Kit (Chemiluminescence)

ELISA: Rat Bad ELISA Kit (Chemiluminescence) [NBP2-69921] -

ELISA: Rat Bad ELISA Kit (Chemiluminescence) [NBP2-69921] - Standard Curve Reference

Kit Contents for Rat Bad ELISA Kit (Chemiluminescence)

Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro CLIA Plate (Dismountable)
Plate Sealer
Product Manual
Reference Standard
Sample Diluent
Substrate Reagent A
Substrate Reagent B

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Bad

Bad is a pro-apoptotic member of the Bcl-2 family. Bad will bind preferentially Bcl-xL over Bcl-2 through its partial (9-16 a.a) BH3 domain. Additionally, Bad can reverse the death repressor activity of Bcl-xL but not of Bcl-2. Upon dephosphorylation, activated Bad translocates to the mitochondria and displace Bcl-2 from tBid sequestration to initiate mitochondrial dysfunction. The switching on/off of its phosphorylation by growth/survival factors regulates Bad activity.

Long Name

Bcl-xL/Bcl-2 Associated Death Promoter

Alternate Names

BBC6, BCL2L8

Gene Symbol

BAD

Additional Bad Products

Product Documents for Rat Bad ELISA Kit (Chemiluminescence)

Download Product Datasheets

Download Manual - Rat Bad ELISA Kit (Chemiluminescence)

Product Specific Notices for Rat Bad ELISA Kit (Chemiluminescence)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.