Human Bad ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-69922

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

75.00 pg/mL

Assay Range

125.00 - 8000 pg/mL
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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

This kit recognizes Human BAD in samples. No significant cross-reactivity or interference between Human BAD and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 4.69%

Inter-Assay Precision (Precision between assays) CV% 5.42%

Recovery for Human Bad ELISA Kit (Colorimetric)

Recovery

85-108%

Linearity

Human Bad ELISA Kit (Colorimetric)

Scientific Data Images for Human Bad ELISA Kit (Colorimetric)

Human Bad ELISA Kit (Colorimetric)

ELISA: Human Bad ELISA Kit (Colorimetric) [NBP2-69922] -

ELISA: Human Bad ELISA Kit (Colorimetric) [NBP2-69922] - Standard Curve Reference

Kit Contents for Human Bad ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Bad

Bad is a pro-apoptotic member of the Bcl-2 family. Bad will bind preferentially Bcl-xL over Bcl-2 through its partial (9-16 a.a) BH3 domain. Additionally, Bad can reverse the death repressor activity of Bcl-xL but not of Bcl-2. Upon dephosphorylation, activated Bad translocates to the mitochondria and displace Bcl-2 from tBid sequestration to initiate mitochondrial dysfunction. The switching on/off of its phosphorylation by growth/survival factors regulates Bad activity.

Long Name

Bcl-xL/Bcl-2 Associated Death Promoter

Alternate Names

BBC6, BCL2L8

Gene Symbol

BAD

Additional Bad Products

Product Documents for Human Bad ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human Bad ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Related Research Areas

Bcl-2 Proteins and Regulators

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