BANF1 Antibody (6J8O2)

Novus Biologicals | Catalog # NBP3-16263

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6J8O2 expressed in HEK293
View all BANF1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-89 of human BANF1 (O75531). MTTSQKHRDFVAEPMGEKPVGSLAGIGEVLGKKLEERGFDKAYVVLGQFLVLKKDEDLFREWLKDTCGANAKQSRDCFGCLREWCDAFL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

10 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BANF1 Antibody (6J8O2)

Western Blot: BANF1 Antibody (6J8O2) [NBP3-16263]

Western Blot: BANF1 Antibody (6J8O2) [NBP3-16263]

Western Blot: BANF1 Antibody (6J8O2) [NBP3-16263] - Western blot analysis of extracts of various cell lines, using BANF1 Rabbit mAb (NBP3-16263) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
BANF1 Antibody (6J8O2)

Immunohistochemistry: BANF1 Antibody (6J8O2) [NBP3-16263] -

Immunohistochemistry: BANF1 Antibody (6J8O2) [NBP3-16263] - Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using BANF1 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
BANF1 Antibody (6J8O2)

Immunohistochemistry: BANF1 Antibody (6J8O2) [NBP3-16263] -

Immunohistochemistry: BANF1 Antibody (6J8O2) [NBP3-16263] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using BANF1 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
BANF1 Antibody (6J8O2)

Immunohistochemistry: BANF1 Antibody (6J8O2) [NBP3-16263] -

Immunohistochemistry: BANF1 Antibody (6J8O2) [NBP3-16263] - Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using BANF1 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
BANF1 Antibody (6J8O2)

Immunohistochemistry: BANF1 Antibody (6J8O2) [NBP3-16263] -

Immunohistochemistry: BANF1 Antibody (6J8O2) [NBP3-16263] - Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using BANF1 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
BANF1 Antibody (6J8O2)

Immunocytochemistry/ Immunofluorescence: BANF1 Antibody (6J8O2) [NBP3-16263] -

Immunocytochemistry/ Immunofluorescence: BANF1 Antibody (6J8O2) [NBP3-16263] - Immunofluorescence analysis of HeLa cells using BANF1 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for BANF1 Antibody (6J8O2)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL.

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:200 - 1:800

Immunohistochemistry-Paraffin

1:200 - 1:800

Western Blot

1:1000 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BANF1

BANF1 is encoded by this gene was identified by its ability to protect retroviruses from intramolecular integration and therefore promote intermolecular integration into the host cell genome. The endogenous function of the protein is unknown. The protein forms a homodimer which localizes to the nucleus and is specifically associated with chromosomes during mitosis. This protein binds to DNA in a non-specific manner and studies in rodents suggest that it also binds to lamina-associated polypeptide 2, a component of the nuclear lamina.

Long Name

Barrier-to-autointegration factor

Alternate Names

BCRG1

Gene Symbol

BANF1

Additional BANF1 Products

Product Documents for BANF1 Antibody (6J8O2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for BANF1 Antibody (6J8O2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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