Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # SAP7F407

Format

BSA Free
View all Bassoon Primary Antibodies »

Product Specifications

Immunogen

Recombinant rat Bassoon.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This affinity purified detects an ~400 kDa protein, corresponding to the apparent molecular mass of Bassoon on SDSPAGE immunoblots, in samples from mouse and rat origins. Additional bands between 97 and 400 kDa may also be detected.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

400 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Bassoon Antibody (SAP7F407) - BSA Free

Western Blot: Bassoon Antibody (SAP7F407) [NB120-13249]

Western Blot: Bassoon Antibody (SAP7F407) [NB120-13249]

Western Blot: Bassoon Antibody (SAP7F407) [NB120-13249] - Rat brain tissue lysate.
Immunocytochemistry/ Immunofluorescence: Bassoon Antibody (SAP7F407) [NB120-13249]

Immunocytochemistry/ Immunofluorescence: Bassoon Antibody (SAP7F407) [NB120-13249]

Immunocytochemistry/Immunofluorescence: Bassoon Antibody (SAP7F407) [NB120-13249] - Rat Hippocampal Neuron, image courtesy of customer.
Immunohistochemistry: Bassoon Antibody (SAP7F407) [NB120-13249]

Immunohistochemistry: Bassoon Antibody (SAP7F407) [NB120-13249]

Immunohistochemistry: Bassoon Antibody (SAP7F407) [NB120-13249] - Bassoon localization in the rat brain cerebellar molecular layer using Bassoon Monoclonal Antibody.

Applications for Bassoon Antibody (SAP7F407) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:400

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

2.5 ug

Western Blot

1:500
Application Notes
Additional bands between 97 and 400 kDa are also identified and are reported to be proteolytic degradation products of Bassoon. Optimal dilutions/concentrations should be determined by the end user. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 28821669).

Reviewed Applications

Read 1 review rated 5 using NB120-13249 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.2) and 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Bassoon

Bassoon is a 420 kDa protein that is a localized at the presynaptic nerve terminals and is believed to play a role in the structural and functional organization of the synaptic vesicle cycle (1-3). Bassoon does not belong to any known protein families. It has been found in rat and mouse with sequence identity of the two proteins at 96% (1). The human BASSOON gene has recently been cloned and localized (3). Bassoon is predicted to contain two double-zinc fingers, three coiled-coil regions, and two polyglutamine domains (1). The polyglutamine domains in the C-terminus are of interest, since it is known that for some human proteins, such as Huntingtin, abnormal amplification of this region can cause late-onset neurodegeneration (1,3). Bassoon is concentrated at sites opposite to postsynaptic densities in synaptic terminals and in cultured neurons, it is found to colocalize with GABA (A) and glutamate (GluR1) receptors (2). Another presynaptic protein, Piccolo (4) was found to colocalize with Bassoon in cultured hippocampal neurons (1). These observations suggested that they serve specific functions at synaptic junctions and may be involved in organization of the cytoskeleton at the site of neurotransmitter release (1).

Alternate Names

bassoon (presynaptic cytomatrix protein)

Entrez Gene IDs

12217 (Mouse); 29138 (Rat)

Gene Symbol

BSN

UniProt

O88778 (Rat)

Additional Bassoon Products

Product Documents for Bassoon Antibody (SAP7F407) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Bassoon Antibody (SAP7F407) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Bassoon Antibody (SAP7F407) - BSA Free

Customer Reviews for Bassoon Antibody (SAP7F407) - BSA Free (1)

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  • Bassoon Antibody (SAP7F407)
    Name: Janosch Heller
    Application: Immunocytochemistry
    Sample Tested: Cultured rat hippocampal neurons
    Species: Rat
    Verified Customer | Posted 03/27/2017
    Fixation: 3% formaldehyde (TAAB) in PBS, 15 minutes RT Blocking: 2% milk powder in PBS, 1 hour RT Permeabilization: 0.2% Triton-X100 Primary Antibody: 1:100 in 1% milk powder in PBS with 0.2% Triton, overnight, 4°C Wash: three times wash with PBS with 0.2% Triton, 10 min each Secondary Antibody: Invitrogen goat anti-mouse Alexa568, 1:500 in PBS with 0.2% Triton, 2 hours, RT Wash: three times wash with PBS, 10 min each Microscope: Confocal (Zeiss LSM710) The antibody labels clusters along the dendrites and somata of the cultured cells as expected.
    Bassoon Antibody (SAP7F407) - BSA Free NB120-13249

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Protocols

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FAQs for Bassoon Antibody (SAP7F407) - BSA Free

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  • Q: I am looking for a good antibody to detect Bassoon in mouse brain slices (PFA fixed); can you recommend any one for this?

    A:

    After searching through our product catalog list, we have found the following antibodies which may be of interest: Bassoo. Using the filters on the left column of the site should allow you to filter by species and desired application. Any antibody that is listed to show validation for use in IHC-P and has also been validated in mouse samples will be backed by our 100% Guarantee. Under the terms and conditions of the guarantee, Novus will provide you with a full refund or replacement should the antibody fail to perform, as specified, in samples expressing the protein.

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