Bax Antibody (6A7) - Azide and BSA Free

Novus Biologicals | Catalog # NBP1-28566

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Monkey

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Functional

Cited:

Immunohistochemistry-Paraffin, Western Blot, Co-IP, Functional Assay, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 6A7

Format

Azide and BSA Free
View all Bax Primary Antibodies »

Product Specifications

Immunogen

KLH-conjugated peptide corresponding to amino acids 12-24 located near the N-terminus common to human, mouse and rat Bax.

Reactivity Notes

Use in Porcine reported in scientific literature (PMID:34243771) Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Specificity

The monoclonal antibody 6A7 reacts with human, mouse and rat Bax but does not bind the soluble cytosolic form of Bax; however, treatment of cells with non-ionic detergents exposes the epitope and allows binding of 6A7 to monomeric forms of Bax but not Bax complexed with either Bcl-2 or Bcl-xL.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Bax Antibody (6A7) - Azide and BSA Free

Western Blot: Bax Antibody (6A7) [NBP1-28566]

Western Blot: Bax Antibody (6A7) [NBP1-28566]

Bax-Antibody-6A7-Western-Blot-NBP1-28566-img0007.jpg
Immunohistochemistry-Frozen: Bax Antibody (6A7) [NBP1-28566]

Immunohistochemistry-Frozen: Bax Antibody (6A7) [NBP1-28566]

Immunohistochemistry-Frozen: Bax Antibody (6A7) [NBP1-28566] - Mouse retina with no pretreatment. IHC-F image submitted by a verified customer review

Western Blot: Bax Antibody (6A7) [NBP1-28566] -

Western Blot: Bax Antibody (6A7) [NBP1-28566] - Analysis of lysates from human metastatic pancreatic cancer cell line L3.6pl using Bax Antibody (clone 6A7; Lot # B168-P780E). This image was submitted via reviews by a verified end user.
Western Blot: Bax Antibody (6A7) [NBP1-28566]

Western Blot: Bax Antibody (6A7) [NBP1-28566]

Western Blot: Bax Antibody (6A7) [NBP1-28566] - Total cell lysates from NIH/3T3 cells were resolved by electrophoresis, transferred to PVDF membrane, and probled with Mouse Anti-Bax UNLB.

Applications for Bax Antibody (6A7) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Frozen

1:10-1:500

Western Blot

< = 1 ug/ml
Application Notes
Use in IHC-P reported in scientific literature (PMID:33143886) Use in functional reported in scientific literature (PMID:31410057). Bax antibody validated for IHC-F, WB from a verified customer reviews.

Reviewed Applications

Read 5 reviews rated 4.8 using NBP1-28566 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

Borate buffered saline, pH 8.2

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Bax

Apoptosis, or programmed cell death, is a well-documented phenomenon in many cellular systems. (2) It plays a key role in tissue and organ development as well as in adult tissues during cell turnover. Apoptosis can be induced by a variety of internal and external stimuli including growth factor deprivation, cytokine treatment, antigen-receptor engagement, cell-cell interactions, irradiation and glucocorticoid treatment. (3) Bax is a member of the Bcl-2 family of apoptosis-associated proteins and, although it is similar in structure to Bcl-2, Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells. Bax targets mitochondrial membranes, inducing mitochondrial damage and subsequent cell death in a caspase-independent manner, presumably via their ion channel-forming activity. These channels may then function to promote a mitochondrial permeability transition or to puncture the outer mitochondrial membrane. In addition to forming homodimer, Bax can heterodimerize with Bcl-2 and Bcl-XL proteins. This heterodimerization between pro- and anti-apoptotic family members may serve a titration function and act as a sensor for the programmed cell death program. (4-13) The monoclonal antibody 2D2 is specific for human Bax. (1)

Long Name

Bcl Associated X Protein

Alternate Names

apoptosis regulator BAX, BCL2-associated X protein, Bcl2-L-4, BCL2L4bcl2-L-4, Bcl-2-like protein 4

Entrez Gene IDs

581 (Human); 12028 (Mouse); 24887 (Rat)

Gene Symbol

BAX

UniProt

Q07812

Additional Bax Products

Product Documents for Bax Antibody (6A7) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Bax Antibody (6A7) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Bcl-2 Proteins and Regulators

Citations for Bax Antibody (6A7) - Azide and BSA Free

Customer Reviews for Bax Antibody (6A7) - Azide and BSA Free (5)

4.8 out of 5
5 Customer Ratings
5 Stars
80%
4 Stars
20%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Bax Antibody (6A7) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 5 of 5 reviews Showing All
Filter By:
Clear Filters X
  • Bax Antibody (6A7) - Azide and BSA Free
    Name: Arpit Kumar
    Application: Western Blot
    Sample Tested: IHC on parrafin sections (mouse) and IHC Sample Tested
    Species: Human, Mouse and Rat
    Verified Customer | Posted 06/17/2026
    Stained sections were observed with a light microscope coupled with a digitally calibrated camera at 10- and 40-fold magnifications. BAX immunoreactivity was graded according to the intensity in NFL, GCL, IPL, INL, OPL, ONL, IS/OS. We performed a semi quantitative scoring method with a typical H-scoring system; which represents staining intensity (0 = no staining; 1 = weak; 2 = moderate; 3 = strong) by the percentage of positive cells in sections. Scores ranged from 0 to 300 (stained intensity multiplied by the percentage of stained cells in percent). Images were quantitatively analyzed for DAB optical density values with ImageJ software in combination with the color deconvolution plug-in in order to differentiate the signals for DAB from hematoxylin. The scale bar of 50 m was applied. Negative control incubations were performed for every experiment, where the primary antibody was replaced by a matching mouse IgG of the same isotype at an equivalent concentration, since none was observed in negative control experiments (in the absence of primary antibody with equivalent concentration matched to mouse IgG isotype negative controls), the staining with BAX signal specificity was thus validated.
    Bax Antibody (6A7) - Azide and BSA Free NBP1-28566
  • Bax Antibody (6A7)
    Name: Sweta Ghosh
    Application: Western Blot
    Sample Tested: Cell lysate from colon cancer cell line and Melanoma cells
    Species: Mouse and Human
    Verified Customer | Posted 04/19/2024
    Bax western blot in cancer cell lysate after apoptosis induction
    Bax Antibody (6A7) - Azide and BSA Free NBP1-28566
  • Bax Antibody (6A7)
    Name: Hayk Simonyan
    Application: Western Blot
    Sample Tested: mouse liver
    Species: Mouse
    Verified Customer | Posted 06/23/2017
    1:1000, liver lysate 10mg
    Bax Antibody (6A7) - Azide and BSA Free NBP1-28566
  • Name: Anonymous
    Application: Western Blot
    Sample Tested:
    Species: Human
    Verified Customer | Posted 03/31/2014
    Bax western blot with Pancreatic cancer cell line L3.6pl.
    Bax Antibody (6A7) - Azide and BSA Free NBP1-28566
  • Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: mouse retina
    Species: Mouse
    Verified Customer | Posted 07/28/2012
    Bax Antibody (6A7) - Azide and BSA Free NBP1-28566

There are no reviews that match your criteria.

Showing  1 - 5 of 5 reviews Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies