Bax Antibody

Novus Biologicals | Catalog # NBP2-67285

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Polyclonal Rabbit IgG
View all Bax Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human Bax aa 1-50 / 192. (SwissProt: Q07812 Human; SwissProt: Q07813 Mouse; SwissProt: Q63690 Rat)

Localization

Mitochondrion membrane, cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit Bax Antibody (NBP2-67285) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Bax Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Bax Antibody

Immunocytochemistry/ Immunofluorescence: Bax Antibody [NBP2-67285]

Immunocytochemistry/ Immunofluorescence: Bax Antibody [NBP2-67285]

Immunocytochemistry/Immunofluorescence: Bax Antibody [NBP2-67285] - Staining Bax in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Bax Antibody [NBP2-67285]

Immunohistochemistry-Paraffin: Bax Antibody [NBP2-67285]

Immunohistochemistry-Paraffin: Bax Antibody [NBP2-67285] - Analysis of paraffin-embedded mouse testis tissue using anti-Bax antibody. Counter stained with hematoxylin.
Immunocytochemistry/ Immunofluorescence: Bax Antibody [NBP2-67285]

Immunocytochemistry/ Immunofluorescence: Bax Antibody [NBP2-67285]

Immunocytochemistry/Immunofluorescence: Bax Antibody [NBP2-67285] - Staining Bax in HepG2 cells (green). The nuclear counter stain is DAPI (blue).Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Bax Antibody [NBP2-67285]

Immunohistochemistry-Paraffin: Bax Antibody [NBP2-67285]

Immunohistochemistry-Paraffin: Bax Antibody [NBP2-67285] - Analysis of paraffin-embedded human cervical tissue using anti-Bax antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Bax Antibody [NBP2-67285]

Immunohistochemistry-Paraffin: Bax Antibody [NBP2-67285]

Immunohistochemistry-Paraffin: Bax Antibody [NBP2-67285] - Analysis of paraffin-embedded human gall bladder tissue using anti-Bax antibody. Counter stained with hematoxylin.
Bax Antibody

Western Blot: Bax Antibody [NBP2-67285] -

Western Blot: Bax Antibody [NBP2-67285] - Analysis of BAX on different lysates with Rabbit anti-BAX antibody at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution.Lane 1: HeLa cell lysateLane 2: MCF7 cell lysateLane 3: HEK-293 cell lysateLysates/proteins at 15 ug/Lane.Predicted band size: 21 kDaObserved band size: 21 kDaExposure time: 1 minute 34 seconds; ECL: K1801;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Bax Antibody

Immunocytochemistry/Immunofluorescence: Bax Antibody [NBP2-67285] -

Immunocytochemistry/Immunofluorescence: Bax Antibody [NBP2-67285] - Analysis of SHSY5Y cells labeling BAX with Rabbit anti-BAX antibody at 1/200 dilution.Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-BAX antibody at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488,) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.Beta Ⅲ tubulin ( red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, were used as the secondary antibody at 1/1,000 dilution.

Applications for Bax Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:200

Immunohistochemistry

1:1,000

Immunohistochemistry-Paraffin

1:1,000

Western Blot

1:5,000-1:20,000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH7.4), 0.2% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Bax

The Bcl-2 family of apoptosis-related genes plays central roles in regulating apoptotic pathways (reviewed in Thomadaki and Scorilas, 2006). Regulation of cell death through apoptosis is critical for the maintenance of homeostasis, defense against infectious agents, and normal development. Bcl-2 family proteins regulate apoptosis primarily through the regulation of mitochondrial outer membrane permeability. In mammals, the family consists of both prosurvival (antiapoptotic) and proapoptotic (prodeath) members. Cellular homeostasis is thought to be dependent on a balance between the actions of prosurvival and proapoptotic proteins. Bcl-2 family proteins can be divided into 3 main subfamilies on the basis of their function and the content of their Bcl-2 homology (BH) domains, for example: 1) Prosurvival: Bcl-2, Bcl-XL, Bcl-W, A1, and Mcl-1 2) Proapoptotic (multidomain): Bax, Bak, and Bok. 3) BH3-only (proapoptotic): Bad, Bcl-XS, Bid, Bik, Bim, Blk, Bmf, Bnip, Noxa, and Puma. Prosurvival members inhibit cells from undergoing apoptosis, whereas proapoptotic and BH3-only subfamily members promote apoptosis. There are 4 BH domains (1-4) conserved among Bcl-2 family proteins. The BH domains are important for function as well as for heterodimerization between family members. Typical prosurvival family members have all four BH domains (1-4), whereas proapoptotic (multidomain) members have BH1, 2 and 3 domains and BH3-only members have only the BH3 domain. Overall, the relative ratio of prosurvival and proapoptotic proteins determines the suseptibility of a cell to various apoptotic stimuli. Many Bcl-2 family proteins are differentially expressed in various malignancies and some are useful prognostic biomarkers. Prosurvival proteins are often elevated in diverse cancers and have the potential to confer resistance to both endogenous cell death stimuli and cancer treatments. Alterations in the ratio or levels of Bcl-2 family proteins have been also associated with nonmalignant diseases including neurodegenerative diseases, autoimmune diseases, AIDs, Down's syndrome, cardiovascular diseases, diabetes, glomerulonephritis, and muscular dystrophy. IMG-5682 recognizes Bax. It reacts with an epitope (PELALDPVPQDASTKKLSE)which is 100% conserved in human Bax isoforms alpha (192 amino acids), beta (218 amino acids), epsilon (164 amino acids), sigma (179 amino acids), and psi (173 amino acids).

Long Name

Bcl Associated X Protein

Alternate Names

apoptosis regulator BAX, BCL2-associated X protein, Bcl2-L-4, BCL2L4bcl2-L-4, Bcl-2-like protein 4

Gene Symbol

BAX

Additional Bax Products

Product Documents for Bax Antibody

Certificate of Analysis

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Product Specific Notices for Bax Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Bcl-2 Proteins and Regulators

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Protocols

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