BCAP31 Antibody (12G12) - BSA Free

Novus Biologicals | Catalog # NBP3-26481

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 12G12 expressed in HEK293

Format

BSA Free
View all BAP31 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human BCAP31 [UniProt P51572]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BCAP31 Antibody (12G12) - BSA Free

BCAP31 Antibody (12G12)

Immunohistochemistry: BCAP31 Antibody (12G12) [NBP3-26481] -

Immunohistochemistry: BCAP31 Antibody (12G12) [NBP3-26481] - Image of BCAP31 Antibody (12G12) diluted at 1:100 and staining in paraffin-embedded human colon cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
BCAP31 Antibody (12G12)

Western Blot: BCAP31 Antibody (12G12) [NBP3-26481] -

Western Blot: BCAP31 Antibody (12G12) [NBP3-26481] - Positive Western Blot detected in: HepG2 whole cell lysate, Ntera-2 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, HEK293 whole cell lysate, A549 whole cell lysate, U251 whole cell lysate, PC3 whole cell lysate, U87 whole cell lysate.
All lanes: BCAP31 Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 28, 35 kDa
Observed band size: 28-36 kDa
BCAP31 Antibody (12G12)

Immunocytochemistry/Immunofluorescence: BCAP31 Antibody (12G12) [NBP3-26481] -

Immunocytochemistry/Immunofluorescence: BCAP31 Antibody (12G12) [NBP3-26481] - Staining of A549 cell with BCAP31 Antibody (12G12) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 526-conjugated Goat Anti-Rabbit IgG (H+L).
BCAP31 Antibody (12G12)

Flow Cytometry: BCAP31 Antibody (12G12) [NBP3-26481] -

Flow Cytometry: BCAP31 Antibody (12G12) [NBP3-26481] - Overlay Peak curve showing PC3 cells stained with BCAP31 Antibody (12G12) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

Applications for BCAP31 Antibody (12G12) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:50-1:200

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: BAP31

BAP31 (B Cell Receptor Associated Protein, 28 kDa) is a multiple membrane spanning protein of the endoplasmic reticulum. It has been shown to form both homo and hetero oligomers with the closely related BAP29, and is a potential apoptotic regulator as a predicted BCL2/BCLXL associated protein. In the C terminal domain of BAP31, there are two identical recognition sites for initiator Caspases 1 and 8 of the programmed death cascade. It is hypothesized that BAP31 plays a role in the transport of selected proteins to the Golgi apparatus. BAP31 has also been shown to control expression of CFTR (cystic fibrosis transmembrane conductance regulator). It is believed that interference with the expression or function of BAP31 in epithelial cells may be a way to circumvent the chloride channel defect in cystic fibrosis.

Long Name

B-cell Receptor-associated Protein 31

Alternate Names

6C6-Ag, BCAP31

Gene Symbol

BCAP31

Additional BAP31 Products

Product Documents for BCAP31 Antibody (12G12) - BSA Free

Certificate of Analysis

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Product Specific Notices for BCAP31 Antibody (12G12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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