Bcl-2 Antibody (OTI9D3)

Novus Biologicals | Catalog # NBP2-46567

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI9D3
View all Bcl-2 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human BCL2 (NP_000624) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

26.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Bcl-2 Antibody (OTI9D3)

Western Blot: Bcl-2 Antibody (OTI9D3) [NBP2-46567]

Western Blot: Bcl-2 Antibody (OTI9D3) [NBP2-46567]

Western Blot: Bcl-2 Antibody (9D3) [NBP2-46567] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCL-2.
Immunohistochemistry: Bcl-2 Antibody (OTI9D3) [NBP2-46567]

Immunohistochemistry: Bcl-2 Antibody (OTI9D3) [NBP2-46567]

Immunohistochemistry: Bcl-2 Antibody (9D3) [NBP2-46567] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Bcl-2 Antibody (OTI9D3) [NBP2-46567]

Immunohistochemistry: Bcl-2 Antibody (OTI9D3) [NBP2-46567]

Immunohistochemistry: Bcl-2 Antibody (9D3) [NBP2-46567] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)
Immunohistochemistry: Bcl-2 Antibody (OTI9D3) [NBP2-46567]

Immunohistochemistry: Bcl-2 Antibody (OTI9D3) [NBP2-46567]

Immunohistochemistry: Bcl-2 Antibody (9D3) [NBP2-46567] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Bcl-2 Antibody (OTI9D3) [NBP2-46567]

Immunohistochemistry: Bcl-2 Antibody (OTI9D3) [NBP2-46567]

Immunohistochemistry: Bcl-2 Antibody (9D3) [NBP2-46567] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Bcl-2 Antibody (OTI9D3) [NBP2-46567]

Immunohistochemistry: Bcl-2 Antibody (OTI9D3) [NBP2-46567]

Immunohistochemistry: Bcl-2 Antibody (9D3) [NBP2-46567] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Bcl-2 Antibody (OTI9D3) [NBP2-46567]

Immunohistochemistry: Bcl-2 Antibody (OTI9D3) [NBP2-46567]

Immunohistochemistry: Bcl-2 Antibody (9D3) [NBP2-46567] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Bcl-2 Antibody (OTI9D3) [NBP2-46567]

Immunohistochemistry: Bcl-2 Antibody (OTI9D3) [NBP2-46567]

Immunohistochemistry: Bcl-2 Antibody (9D3) [NBP2-46567] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Bcl-2 Antibody (OTI9D3) [NBP2-46567]

Immunohistochemistry: Bcl-2 Antibody (OTI9D3) [NBP2-46567]

Immunohistochemistry: Bcl-2 Antibody (9D3) [NBP2-46567] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Bcl-2 Antibody (OTI9D3) [NBP2-46567]

Immunohistochemistry: Bcl-2 Antibody (OTI9D3) [NBP2-46567]

Immunohistochemistry: Bcl-2 Antibody (9D3) [NBP2-46567] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Bcl-2 Antibody (OTI9D3) [NBP2-46567]

Immunohistochemistry: Bcl-2 Antibody (OTI9D3) [NBP2-46567]

Immunohistochemistry: Bcl-2 Antibody (9D3) [NBP2-46567] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Bcl-2 Antibody (OTI9D3) [NBP2-46567]

Immunohistochemistry: Bcl-2 Antibody (OTI9D3) [NBP2-46567]

Immunohistochemistry: Bcl-2 Antibody (9D3) [NBP2-46567] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Applications for Bcl-2 Antibody (OTI9D3)

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Bcl-2

The Bcl-2 family of proteins regulate apoptosis by controlling mitochondrial permeability and release of cytochrome c. Bcl-2 is an anti-apoptotic protein that resides in the outer mitochondrial wall and inhibits release of cytochrome c (1). Over-expression of Bcl-2 has been shown to promote cell survival by suppressing apoptosis. It has been documented that bcl-2 becomes deregulated in tumor cells as a result of translocation into the immunoglobulin heavy-chain locus and is therefore activated in B cell malignancies (2). It has also been shown that post-translational modification of Bcl-2, phosphorylation at serine 70, may be required to render its full and/or enhanced anti-apoptotic effect (3, 4).

Long Name

B Cell Lymphoma/Leukemia 2

Alternate Names

Bcl2

Gene Symbol

BCL2

Additional Bcl-2 Products

Product Documents for Bcl-2 Antibody (OTI9D3)

Certificate of Analysis

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Product Specific Notices for Bcl-2 Antibody (OTI9D3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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