Bcl 7A Antibody

Name: Bcl 7A Antibody
Brand: Novus Biologicals
SKU: NB100-769
Price: 399 USD
Availability: InStock

Novus Biologicals | Catalog # NB100-769

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Product Documents

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

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Product Specifications

Immunogen

Peptide with sequence C-MKLEASQQNSEEM corresponding to C-Terminus according to NP_066273.1, NP_001019979.1.

Specificity

This antibody is expected to recognize both reported isoforms (NP_066273.1; NP_001019979.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Bcl 7A Antibody

Western Blot: Bcl 7A Antibody [NB100-769]

Western Blot: Bcl 7A Antibody [NB100-769] - Staining of Human Lymphnode lysate (RIPA buffer, 35 ug total protein per lane). Antibody at 1 ug/mL. Detected by chemiluminescence.

Immunocytochemistry/ Immunofluorescence: Bcl 7A Antibody [NB100-769]

Immunocytochemistry/Immunofluorescence: Bcl 7A Antibody [NB100-769] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Flow Cytometry: Bcl 7A Antibody [NB100-769]

Flow Cytometry: Bcl 7A Antibody [NB100-769] - Flow cytometric analysis of paraformaldehyde fixed Daudi cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for Bcl 7A Antibody

Application

Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:4000

Western Blot

1 - 3 ug/mL

Application Notes

WB: Approx. 26 kDa band observed in human lymph node lysates (calculated MW of 25.0 kDa band according to NP_066273.1).

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Bcl 7A

This gene is directly involved--with Myc and IgH--in a three-way gene translocation in a Burkitt lymphoma cell line. As a result of the gene translocation, the N-terminal region of the gene product is disrupted, which is thought to be related to the pathogenesis of a subset of high-grade B cell non-Hodgkin lymphoma. The N-terminal segment involved in the translocation includes the region that shares a strong sequence similarity with those of BCL7B and BCL7C.

Alternate Names

B-cell CLL/lymphoma 7 protein family member A, B-cell CLL/lymphoma 7A, B-cell CLL/lymphoma-7, BCL7

Entrez Gene IDs

605 (Human); 77045 (Mouse)

Gene Symbol

BCL7A

UniProt

NP_066273 (Human)

Additional Bcl 7A Products

Product Documents for Bcl 7A Antibody

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Certificate of Analysis

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Product Specific Notices for Bcl 7A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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