BCL-W/BCL2L2 Antibody (4G12E6) - BSA Free

Novus Biologicals | Catalog # NBP2-61706

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 4G12E6

Format

BSA Free
View all Bcl-w Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human BCL-W/BCL2L2 (AA: 6-118) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

20.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BCL-W/BCL2L2 Antibody (4G12E6) - BSA Free

Western Blot: BCL-W/BCL2L2 Antibody (4G12E6)BSA Free [NBP2-61706]

Western Blot: BCL-W/BCL2L2 Antibody (4G12E6)BSA Free [NBP2-61706]

Western Blot: BCL-W/BCL2L2 Antibody (4G12E6) [NBP2-61706] - Analysis using BCL2L2 mAb against HEK293 (1) and BCL2L2 (AA: 6-118)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: BCL-W/BCL2L2 Antibody (4G12E6) - BSA Free [NBP2-61706]

Immunocytochemistry/ Immunofluorescence: BCL-W/BCL2L2 Antibody (4G12E6) - BSA Free [NBP2-61706]

Immunocytochemistry/Immunofluorescence: BCL-W/BCL2L2 Antibody (4G12E6) [NBP2-61706] - Analysis of Hela cells using BCL2L2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Immunohistochemistry: BCL-W/BCL2L2 Antibody (4G12E6) - BSA Free [NBP2-61706]

Immunohistochemistry: BCL-W/BCL2L2 Antibody (4G12E6) - BSA Free [NBP2-61706]

Immunohistochemistry: BCL-W/BCL2L2 Antibody (4G12E6) [NBP2-61706] - Analysis of paraffin-embedded ovarian cancer tissues using BCL2L2 mouse mAb with DAB staining.
Flow Cytometry: BCL-W/BCL2L2 Antibody (4G12E6) - BSA Free [NBP2-61706]

Flow Cytometry: BCL-W/BCL2L2 Antibody (4G12E6) - BSA Free [NBP2-61706]

Flow Cytometry: BCL-W/BCL2L2 Antibody (4G12E6) [NBP2-61706] - Analysis of K562 cells using BCL2L2 mouse mAb (green) and negative control (red).
Western Blot: BCL-W/BCL2L2 Antibody (4G12E6)BSA Free [NBP2-61706]

Western Blot: BCL-W/BCL2L2 Antibody (4G12E6)BSA Free [NBP2-61706]

Western Blot: BCL-W/BCL2L2 Antibody (4G12E6) [NBP2-61706] - Analysis using BCL2L2 mAb against human BCL2L2 (AA: 6-118) recombinant protein. (Expected MW is 38.2 kDa)
Western Blot: BCL-W/BCL2L2 Antibody (4G12E6)BSA Free [NBP2-61706]

Western Blot: BCL-W/BCL2L2 Antibody (4G12E6)BSA Free [NBP2-61706]

Western Blot: BCL-W/BCL2L2 Antibody (4G12E6) [NBP2-61706] - Analysis using BCL2L2 mouse mAb against HCT116 (1), LOVO (2), SW480 (3), and HL-60 (4) cell lysate.
Immunohistochemistry: BCL-W/BCL2L2 Antibody (4G12E6) - BSA Free [NBP2-61706]

Immunohistochemistry: BCL-W/BCL2L2 Antibody (4G12E6) - BSA Free [NBP2-61706]

Immunohistochemistry: BCL-W/BCL2L2 Antibody (4G12E6) [NBP2-61706] - Analysis of paraffin-embedded cervical cancer tissues using BCL2L2 mouse mAb with DAB staining.
Flow Cytometry: BCL-W/BCL2L2 Antibody (4G12E6) - BSA Free [NBP2-61706]

Flow Cytometry: BCL-W/BCL2L2 Antibody (4G12E6) - BSA Free [NBP2-61706]

Flow Cytometry: BCL-W/BCL2L2 Antibody (4G12E6) [NBP2-61706] - Analysis of Hela cells using BCL2L2 mouse mAb (green) and negative control (red).
ELISA: BCL-W/BCL2L2 Antibody (4G12E6) - BSA Free [NBP2-61706]

ELISA: BCL-W/BCL2L2 Antibody (4G12E6) - BSA Free [NBP2-61706]

ELISA: BCL-W/BCL2L2 Antibody (4G12E6) [NBP2-61706] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Applications for BCL-W/BCL2L2 Antibody (4G12E6) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Bcl-w

This gene encodes a member of the BCL-2 protein family. The proteins of this family form hetero- or homodimers and act as anti- and pro-apoptotic regulators. Expression of this gene in cells has been shown to contribute to reduced cell apoptosis under cytotoxic conditions. Studies of the related gene in mice indicated a role in the survival of NGF- and BDNF-dependent neurons. Mutation and knockout studies of the mouse gene demonstrated an essential role in adult spermatogenesis. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream PABPN1 (poly(A) binding protein, nuclear 1) gene.

Long Name

B Cell Lymphoma/Leukemia w

Alternate Names

BCL2L2, Bclw

Gene Symbol

BCL2L2

Additional Bcl-w Products

Product Documents for BCL-W/BCL2L2 Antibody (4G12E6) - BSA Free

Certificate of Analysis

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Product Specific Notices for BCL-W/BCL2L2 Antibody (4G12E6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Bcl-2 Proteins and Regulators

Citations for BCL-W/BCL2L2 Antibody (4G12E6) - BSA Free

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Protocols

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FAQs

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