Bcl-xL Antibody (OTI4A9)

Novus Biologicals | Catalog # NBP1-47665

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI4A9
View all Bcl-xL Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment expressed in E.coli corresponding to amino acids 1-212 of human Bcl-XL

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens BCL2-like 1 (BCL2L1), nuclear gene encoding mitochondrial protein, transcript variant 1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

25.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Bcl-xL Antibody (OTI4A9)

Western Blot: Bcl-xL Antibody (OTI4A9) [NBP1-47665]

Western Blot: Bcl-xL Antibody (OTI4A9) [NBP1-47665]

Western Blot: Bcl-xL Antibody (4A9) [NBP1-47665] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using antiBCL-XL monoclonal antibody.(1:200)
Immunocytochemistry/ Immunofluorescence: Bcl-xL Antibody (OTI4A9) [NBP1-47665]

Immunocytochemistry/ Immunofluorescence: Bcl-xL Antibody (OTI4A9) [NBP1-47665]

Immunocytochemistry/Immunofluorescence: Bcl-Xl Antibody (4A9) [NBP1-47665] - Bcl-Xl Antibody (4A9) Staining of COS7 cells transiently transfected by pCMV6-ENTRY BCL2L1.
Immunohistochemistry-Paraffin: Bcl-xL Antibody (OTI4A9) [NBP1-47665]

Immunohistochemistry-Paraffin: Bcl-xL Antibody (OTI4A9) [NBP1-47665]

Immunohistochemistry-Paraffin: Bcl-xL Antibody (4A9) [NBP1-47665] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-BCL-XL mouse monoclonal antibody.
Western Blot: Bcl-xL Antibody (OTI4A9) [NBP1-47665]

Western Blot: Bcl-xL Antibody (OTI4A9) [NBP1-47665]

Western Blot: Bcl-Xl Antibody (4A9) [NBP1-47665] - Bcl-Xl Antibody (4A9) HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCL2L1(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCL2L1.
Western Blot: Bcl-xL Antibody (OTI4A9) [NBP1-47665]

Western Blot: Bcl-xL Antibody (OTI4A9) [NBP1-47665]

Western Blot: Bcl-xL Antibody (4A9) [NBP1-47665] - Analysis of extracts (10ug) from 3 different cell lines by using anti-BCL-XL monoclonal antibody.(1:200)
Immunocytochemistry/ Immunofluorescence: Bcl-xL Antibody (OTI4A9) [NBP1-47665]

Immunocytochemistry/ Immunofluorescence: Bcl-xL Antibody (OTI4A9) [NBP1-47665]

Immunocytochemistry/Immunofluorescence: Bcl-Xl Antibody (4A9) [NBP1-47665] - Bcl-Xl Antibody (4A9) Immunofluorescent staining of HT29 cells using anti-BCL2L1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Bcl-xL Antibody (OTI4A9) [NBP1-47665]

Immunohistochemistry-Paraffin: Bcl-xL Antibody (OTI4A9) [NBP1-47665]

Immunohistochemistry-Paraffin: Bcl-xL Antibody (4A9) [NBP1-47665] - Staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-BCL-XL mouse monoclonal antibody.

Applications for Bcl-xL Antibody (OTI4A9)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:200 - 1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA, 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.25 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Bcl-xL

Bcl-XL belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator

Long Name

B Cell Lymphoma/Leukemia x Long Form

Alternate Names

BclxL

Entrez Gene IDs

598 (Human)

Gene Symbol

BCL2L1

Additional Bcl-xL Products

Product Documents for Bcl-xL Antibody (OTI4A9)

Certificate of Analysis

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Product Specific Notices for Bcl-xL Antibody (OTI4A9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Bcl-xL Antibody (OTI4A9)

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Protocols

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