Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Alligator, Canine, Primate

Cited:

Human, Mouse, Rat, Porcine, Alligator, Canine, Primate

Predicted:

Bovine (97%), Chicken (91%), Orangutan (99%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Knockdown Validated

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Beclin 1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to an internal portion of human Beclin 1 (within residues 150-300). [Swiss-Prot: Q14457]

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34622072). Canine reactivity reported in scientific literature (PMID: 24027311). Use in Rat reported in scientific literature (PMID:32501746).

Localization

Cell Membrane, Cytoplasmic and Golgi Apparatus. Expressed in dendrites and cell bodies of cerebellar Purkinje cells

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Beclin 1 Antibody - BSA Free

Simple Western: Beclin 1 AntibodyBSA Free [NB110-87318]

Simple Western: Beclin 1 AntibodyBSA Free [NB110-87318]

Simple Western: Beclin 1 Antibody [NB110-87318] - Lane view shows a specific band for Beclin in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Western Blot: Beclin 1 AntibodyBSA Free [NB110-87318]

Western Blot: Beclin 1 AntibodyBSA Free [NB110-87318]

Western Blot: Beclin 1 Antibody [NB110-87318] - Total protein from human HeLa and mouse Neuro2A cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Beclin-1 in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Immunohistochemistry-Paraffin: Beclin 1 Antibody - BSA Free [NB110-87318]

Immunohistochemistry-Paraffin: Beclin 1 Antibody - BSA Free [NB110-87318]

Immunohistochemistry-Paraffin: Beclin 1 Antibody [NB110-87318] - Analysis of a FFPE tissue section of human breast cancer using Beclin 1 antibody (Lot 71917) at 1:300 dilution. The primary antibody bound to Beclin 1 antigen in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. Cytoplasmic staining can be seen in the cancer as well as the stromal cells. The signal was strongest in a subset of stromal cells which appears to be the cancer associated fibroblasts.
Western Blot: Beclin 1 AntibodyBSA Free [NB110-87318]

Western Blot: Beclin 1 AntibodyBSA Free [NB110-87318]

Beclin-1-Antibody-Western-Blot-NB110-87318-img0021.jpg
Immunocytochemistry/ Immunofluorescence: Beclin 1 Antibody - BSA Free [NB110-87318]

Immunocytochemistry/ Immunofluorescence: Beclin 1 Antibody - BSA Free [NB110-87318]

Immunocytochemistry/Immunofluorescence: Beclin 1 Antibody [NB110-87318] - Beclin 1/ATG6 Antibody [NB110-87318] - Beclin1 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Immunohistochemistry-Paraffin: Beclin 1 Antibody - BSA Free [NB110-87318]

Immunohistochemistry-Paraffin: Beclin 1 Antibody - BSA Free [NB110-87318]

Immunohistochemistry-Paraffin: Beclin 1 Antibody [NB110-87318] - Analysis of a FFPE tissue section of human breast cancer using Beclin 1 antibody (Lot F4) at 1:300 dilution. The primary antibody bound to Beclin 1 antigen in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. Cytoplasmic staining can be seen in the cancer as well as the stromal cells. The signal was strongest in a subset of stromal cells which appears to be the cancer associated fibroblasts.
Western Blot: Beclin 1 AntibodyBSA Free [NB110-87318]

Western Blot: Beclin 1 AntibodyBSA Free [NB110-87318]

Western Blot: Beclin 1 Antibody [NB110-87318] - Image from verified customer review.
Immunohistochemistry: Beclin 1 Antibody - BSA Free [NB110-87318]

Immunohistochemistry: Beclin 1 Antibody - BSA Free [NB110-87318]

Immunohistochemistry: Beclin 1/ATG6 Antibody [NB110-87318] - Staining of normal breast tissue.
Beclin 1 Antibody - BSA Free

Western Blot: Beclin 1 Antibody - BSA Free [NB110-87318] -

Western Blot: Beclin 1 Antibody - BSA Free [NB110-87318] - Maintenance of basal levels of autophagy in mice overexpressing htt fragments in the hypothalamus.The expression levels of selected autophagy markers were analyzed by Western blot in mice bilaterally injected with rAAV5-htt853-18Q or rAAV5-htt853-79Q & compared to uninjected controls 8 weeks post injection (n = 6–7/group). The data displayed maintenance of the autophagy flux, despite the presence of a clear metabolic phenotype in the injected mice. The data in the graphs are expressed as mean ± SEM relative to the uninjected controls. The Western blots are representative of one sample for each group. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0083050), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Beclin 1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50

Immunohistochemistry

1:50 - 1:400

Immunohistochemistry-Paraffin

1:50 - 1:400

Knockdown Validated

reported in scientific literature (PMID 29297744)

Simple Western

1:12.5

Western Blot

1:1000
Application Notes
In Western blot, a band is seen approx. 51 kDa. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate 1.0 mg/mL, separated by Size, antibody dilution of 1:12.5, apparent MW was 59 kDa

Reviewed Applications

Read 4 reviews rated 4.8 using NB110-87318 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Beclin 1

Beclin 1 is the first identified mammalian gene to mediate autophagy and also has tumor suppressor and antiviral function. Autophagy, a process of bulk protein degradation through an autophagosomic-lysosomal pathway, is important for differentiation, survival during nutrient deprivation, and normal growth control, and is often defective in tumor cells. Beclin 1 was originally isolated in a yeast two hybrid screen to identify Bcl-2-binding partners and maps to a tumor susceptibility locus on human chromosome 17q21 that is frequently monoallelically deleted in human breast, ovarian and prostate cancer. Beclin 1 encodes an evolutionarily conserved 60 kDa coiled coil protein that is expressed in human muscle, epithelial cells and neurons. In gene transfer studies, Beclin 1 promotes nutrient deprivation-induced autophagy, inhibits mammary tumorigenesis, and inhibits viral replication. Expression of the Beclin 1 protein is frequently decreased in malignant breast epithelial cells. Based upon these observations, it is speculated that beclin 1 may work through induction of autophagy to negatively regulate tumorigenesis and to control viral infections. Beclin 1 may also play a role in other biological processes in which autophagy is important such as cell differentiation and nutritional stress responses.

Long Name

Beclin1, Autophagy-related

Alternate Names

ATG6, BECN1, GT197, VPS30

Gene Symbol

BECN1

Additional Beclin 1 Products

Product Documents for Beclin 1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Beclin 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Autophagy

Citations for Beclin 1 Antibody - BSA Free

Customer Reviews for Beclin 1 Antibody - BSA Free (4)

4.8 out of 5
4 Customer Ratings
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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • Beclin 1 Antibody
    Name: Mathumai Kanapathipillai
    Application: Western Blot
    Sample Tested: A549 human lung carcinoma cell line
    Species: Human
    Verified Customer | Posted 03/03/2017
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Human microglia cell lysate
    Species: Human
    Verified Customer | Posted 04/25/2014
    Beclin1
    Beclin 1 Antibody - BSA Free NB110-87318
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Human
    Species: Human
    Verified Customer | Posted 11/21/2011
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Mouse
    Species: Mouse
    Verified Customer | Posted 11/21/2011

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Protocols

View specific protocols for Beclin 1 Antibody - BSA Free (NB110-87318):


Western Blot Protocol

1. Perform SDS-PAGE (4-12% MOPS) on samples to be analyzed, loading 40 ug of total protein per lane.
2. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer
apparatus.
3. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
4. Rinse the blot in TBS for approximately 5 minutes.
5. Block the membrane using 5% BSA in TBS + Tween, 1 hour at RT.
6. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each.
7. Dilute the rabbit anti-Beclin1 primary antibody (NB 110-87318) in blocking buffer and incubate 1 hour at room temperature.
8. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each.
9. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturers
instructions) and incubate 1 hour at room temperature.
10. Wash the blot in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).

Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2%, provided it does not interfere with antibody-antigen binding.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Beclin 1 Antibody - BSA Free

Showing  1 - 2 of 2 FAQs Showing All

  • Q: Can this NB110-87318 antibody applied to hamster?

    A: The Beclin 1 Antibody, NB110-87318, has been validated in the following species: Human, mouse, rat, porcine, monkey, bovine, chicken, porcine and Xenopus. Hamster is not one of the species tested and validated and therefore we cannot guarantee that it will work in that species. If you are interested in testing this antibody in hamster, please take a look at our Innovators Reward Program.

  • Q: We found the tested applications of NB110-87318 including IHC and IHC-P. Does it mean only IHC with paraffin embeded sections were tested with this antibody, and thus it may not work on frozen sections?

    A: Our Beclin 1 antibody with catalogue number NB110-87318 has been validated for IHC with paraffin-embedded samples, but has not yet been tested with frozen tissue. We use the term IHC as a general search term, then this is further refined as IHC-P (paraffin-embedded) or IHC-Fr (frozen).

  • Q: Can this NB110-87318 antibody applied to hamster?

    A: The Beclin 1 Antibody, NB110-87318, has been validated in the following species: Human, mouse, rat, porcine, monkey, bovine, chicken, porcine and Xenopus. Hamster is not one of the species tested and validated and therefore we cannot guarantee that it will work in that species. If you are interested in testing this antibody in hamster, please take a look at our Innovators Reward Program.

  • Q: We found the tested applications of NB110-87318 including IHC and IHC-P. Does it mean only IHC with paraffin embeded sections were tested with this antibody, and thus it may not work on frozen sections?

    A: Our Beclin 1 antibody with catalogue number NB110-87318 has been validated for IHC with paraffin-embedded samples, but has not yet been tested with frozen tissue. We use the term IHC as a general search term, then this is further refined as IHC-P (paraffin-embedded) or IHC-Fr (frozen).

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Associated Pathways

Akt Signaling Pathway Akt Signaling Pathway Thumbnail