beta Adducin Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-33974

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all beta Adducin Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: NNSSNIWALRQIADFMASTSHAVFPTSSMNVSMMTPINDLHTADSLNLAKGERLMRCKISSVYRLLDLYGWAQLSDTYVTLRVSKEQDHF

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for beta Adducin Antibody - BSA Free

Western Blot: beta Adducin Antibody [NBP2-33974]

Western Blot: beta Adducin Antibody [NBP2-33974]

Western Blot: beta Adducin Antibody [NBP2-33974] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunohistochemistry-Paraffin: beta Adducin Antibody [NBP2-33974]

Immunohistochemistry-Paraffin: beta Adducin Antibody [NBP2-33974]

Immunohistochemistry-Paraffin: beta Adducin Antibody [NBP2-33974] - Staining in human cerebral cortex and pancreas tissues using anti-ADD2 antibody. Corresponding ADD2 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: beta Adducin Antibody [NBP2-33974]

Immunohistochemistry-Paraffin: beta Adducin Antibody [NBP2-33974]

Immunohistochemistry-Paraffin: beta Adducin Antibody [NBP2-33974] - Staining of human cerebral cortex shows high expression.
Immunohistochemistry-Paraffin: beta Adducin Antibody [NBP2-33974]

Immunohistochemistry-Paraffin: beta Adducin Antibody [NBP2-33974]

Immunohistochemistry-Paraffin: beta Adducin Antibody [NBP2-33974] - Staining of human pancreas shows low expression as expected.

Applications for beta Adducin Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended. See Simple Western Antibody Database for Simple Western validation: Separated by size; matrix was 12-230 kDa; detected by Chemiluminescence.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: beta Adducin

Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta and gamma). Adducin is a protein associated with the inner leaflet of the plasma membrane and is one of the proteins localized at the spectrin-actin junction of the membrane skeleton. The cortical actin cytoskeletal network is lost during apoptosis and adducins are central in the cortical actin network organization. Adducin alpha is a cytoskeletal protein involved with sodium-pump activity in the renal tubule and is associated with hypertension. The expression of Adducin alpha and Adducin gamma is ubiquitous in contrast to the restricted expression of Adducin beta. Adducin beta is expressed at high levels in brain and hematopoietic tissues, such as bone marrow in humans, and spleen in mice.

Alternate Names

ADDBErythrocyte adducin subunit beta, adducin 2 (beta), beta adducin, beta-adducin

Gene Symbol

ADD2

UniProt

P35612 (Human)

Additional beta Adducin Products

Product Documents for beta Adducin Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for beta Adducin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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