beta Amyloid [Pyro Glu11] Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP1-44070

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Drosophila, Primate

Cited:

Insect - Drosophila

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA, Immunoassay, Immunocytochemistry/ Immunofluorescence

Cited:

Immunoassay

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Amyloid beta Primary Antibodies »

Product Specifications

Immunogen

beta Amyloid [Pyro Glu11] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the N-terminus 11-pyro E start point of human beta Amyloid. (Uniprot: P05067)

Reactivity Notes

Drosophila reactivity reported in scientific literature (PMID: 26208119)

Modification

Pyro Glu11

Specificity

This antibody is specific to the N-terminus 11-pyro E start point.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for beta Amyloid [Pyro Glu11] Antibody - Azide and BSA Free

Immunohistochemistry: beta Amyloid [Pyro Glu11] Antibody [NBP1-44070]

Immunohistochemistry: beta Amyloid [Pyro Glu11] Antibody [NBP1-44070]

Immunohistochemistry: beta Amyloid [Pyro Glu11] Antibody [NBP1-44070] - beta-amyloid (pyroglu11) NBP1-44070 in red (left) co-labeled with 6E10 in green (middle) and merge (right). Image from verified customer review.
Western Blot: beta Amyloid [Pyro Glu11] Antibody [NBP1-44070]

Western Blot: beta Amyloid [Pyro Glu11] Antibody [NBP1-44070]

Western Blot: beta Amyloid [Pyro Glu11] Antibody [NBP1-44070] - Lane 1: Ovalbumin Control. Lane 2: beta Amyloid pyro E11 peptide. Lane 3: beta Amyloid 11 peptide. Lane 4: beta Amyloid pyro E3 peptide. Load: 1 ug per lane. Primary antibody: Beta Amyloid pyro E11 antibody at 0.2ug/mL for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 86.9 kDa for beta Amyloid pyro E11 peptide.
Immunohistochemistry: beta Amyloid [Pyro Glu11] Antibody [NBP1-44070]

Immunohistochemistry: beta Amyloid [Pyro Glu11] Antibody [NBP1-44070]

Immunohistochemistry: beta Amyloid [Pyro Glu11] Antibody [NBP1-44070] - Laser scanning confocal microscopy was carried out using human tissue sections from frontal cortex. Figure A shows an example section that was immunostained for the presence of pyrE11Abeta (green). Figure B shows that the immunogen peptide completely blocked the NBP1-44070 antibody.

Applications for beta Amyloid [Pyro Glu11] Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

1:20000-1:60000

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry

1:100-1:500

Western Blot

0.2 ug/ml
Application Notes
This product has been tested by western blot and is useful for ELISA, Western Blot and Immunostaining. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~86.9kDa corresponding to the appropriate cell lysate or extract.

Use in Immunoassay reported in scientific literature (PMID: 26208119).

Reviewed Applications

Read 1 review rated 4 using NBP1-44070 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 30% Glycerol

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Amyloid beta

Beta amyloid, also known as Abeta, is a peptide that varies in length from 39 to 43 amino acids and is processed from the transmembrane protein amyloid precursor protein (APP) by beta-secretase (BACE-1) and gamma-secretase. Beta amyloid [1-40], beta amyloid [1-42], and beta amyloid [1-43] peptides result from cleavage of APP after residues 40, 42, and 43, respectively. The aggregation of beta amyloid monomers produces insoluble oligomers and protofibrils. The longer "stickier" forms of beta amyloid, particularly ABeta42, are known as the main component of amyloid plaques, which are deposits (senile plaques or neuritic plaques) found in the brains of patients with Alzheimer's disease (AD) (1,2). Research indicates that intraneuronal beta amyloid accumulation may be an important proximal neurotoxic event in the pathogenesis of Alzheimer's disease. Abeta deposits have also been reported in the hearts of AD patients (3).

Pyroglutamate amyloid beta peptides (pGlu-Abeta) are N-terminal truncations in which the N-terminal glutamate is cyclized to pyroglutamate resulting in pGlu-Abeta (3-40/42 and 11-40/42) (4). This pyrE modification may have a greater propensity to aggregate under physiological conditions and has been implicated as the molecular species responsible for seeding larger oligomers of amyloid beta.

References

1. Chen GF, Xu TH, Yan Y, Zhou YR, Jiang Y, Melcher K, Xu HE. (2017) Amyloid beta: structure, biology and structure-based therapeutic development. Acta Pharmacol Sin. 38(9):1205-1235. PMID: 28713158

2. De-Paula VJ1, Radanovic M, Diniz BS, Forlenza OV. (2012) Alzheimer's disease. Subcell Biochem. 65:329-52. PMID: 23225010

3. Schaich CL, Maurer MS, Nadkarni NK. (2019) Amyloidosis of the Brain and Heart: Two Sides of the Same Coin? JACC Heart Fail. 7(2):129-131. PMID: 30704604

4. He W, Barrow CJ. (1999) The A beta 3-pyroglutamyl and 11-pyroglutamyl peptides found in senile plaque have greater beta-sheet forming and aggregation propensities in vitro than full-length A beta. Biochemistry. 38(33):10871-7. PMID: 10451383

Alternate Names

Abeta, beta-Amyloid Peptide

Entrez Gene IDs

351 (Human)

Gene Symbol

APP

UniProt

P05067

Additional Amyloid beta Products

Product Documents for beta Amyloid [Pyro Glu11] Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for beta Amyloid [Pyro Glu11] Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for beta Amyloid [Pyro Glu11] Antibody - Azide and BSA Free

Customer Reviews for beta Amyloid [Pyro Glu11] Antibody - Azide and BSA Free (1)

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Customer Images

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  • Name: Sam Marsh
    Application: Immunofluorescence
    Sample Tested: Transgenic Mouse Brain (40um)
    Species: Mouse
    Verified Customer | Posted 03/21/2014
    beta-amyloid (pyroglu11) NBP1-44070 in red (left) co-labeled with 6E10 in green (middle) and merge (right).
    beta Amyloid [Pyro Glu11] Antibody - Azide and BSA Free NBP1-44070

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