beta-Arrestin 2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-24569

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine, Canine, Chicken, Equine, Primate, Xenopus, Zebrafish

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all beta-Arrestin 2 Primary Antibodies »

Product Specifications

Immunogen

A peptide sequence corresponding to an area between amino acids 1 and 50 of human ARRB2 was used as the immunogen for the antibody, Gen Bank no. gb:ABG47460.1.

Reactivity Notes

Chimpanzee, platypus, opssoum, boar (100%), Tetradon (94%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for beta-Arrestin 2 Antibody - BSA Free

Western Blot: beta-Arrestin 2 AntibodyBSA Free [NBP2-24569]

Western Blot: beta-Arrestin 2 AntibodyBSA Free [NBP2-24569]

Western Blot: beta-Arrestin 2 Antibody [NBP2-24569] - Analysis of ARRB2 in HUVEC cell lysate in the 1) absence and 2) presence of immunizing peptide using ARRB2 antibody at 0.25 ug/mL. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used.
Immunohistochemistry-Paraffin: beta-Arrestin 2 Antibody - BSA Free [NBP2-24569]

Immunohistochemistry-Paraffin: beta-Arrestin 2 Antibody - BSA Free [NBP2-24569]

Immunohistochemistry-Paraffin: beta-Arrestin 2 Antibody [NBP2-24569] - Human thyroid tissue stained with ARRB2 antibody at 5 ug/mL. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Simple Western: beta-Arrestin 2 AntibodyBSA Free [NBP2-24569]

Simple Western: beta-Arrestin 2 AntibodyBSA Free [NBP2-24569]

Simple Western: beta-Arrestin 2 Antibody [NBP2-24569] - Simple Western lane view shows a specific band for beta Arrestin in 0.5 mg/mL of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Simple Western: beta-Arrestin 2 AntibodyBSA Free [NBP2-24569]

Simple Western: beta-Arrestin 2 AntibodyBSA Free [NBP2-24569]

Simple Western: beta-Arrestin 2 Antibody [NBP2-24569] - Beta-arrestin antibody detects 2 peaks at 32 and 45 kDa using Simple Western. Traditional size of beta-arrestin 2 is ~55 kDa. Image from verified customer review.

Applications for beta-Arrestin 2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

5 ug/mL

Simple Western

1:100

Western Blot

0.25 - 0.5 ug/mL
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in Jurkat lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:100, apparent MW was 54 kDa

Reviewed Applications

Read 1 review rated 3 using NBP2-24569 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: beta-Arrestin 2

Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 2, like arrestin beta 1, was shown to inhibit beta-adrenergic receptor function in vitro. It is expressed at high levels in the central nervous system and may play a role in the regulation of synaptic receptors. Besides the brain, a cDNA for arrestin beta 2 was isolated from thyroid gland, and thus it may also be involved in hormone-specific desensitization of TSH receptors. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined.

Alternate Names

ARB2, ARR2, ARRB2, betaArrestin 2

Gene Symbol

ARRB2

Additional beta-Arrestin 2 Products

Product Documents for beta-Arrestin 2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for beta-Arrestin 2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for beta-Arrestin 2 Antibody - BSA Free

Customer Reviews for beta-Arrestin 2 Antibody - BSA Free (1)

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  • beta-Arrestin 2 Antibody
    Name: Anonymous
    Application: Simple Western
    Sample Tested: Mouse skeletal muscle
    Species: Mouse
    Verified Customer | Posted 11/16/2018
    Beta-arrestin antibody detects 2 peaks at 32 and 45 kDa using Simple Western. Traditional size of beta-arrestin 2 is ~55 kDa.
    antibody used at 1:50 dilution 0.5 mg/ml protein concentration of the sample GAPDH, 1:150 dilution, (14C10, HRP Conjugate, #3683 CST) used as loading control (~39 kDa)
    beta-Arrestin 2 Antibody - BSA Free NBP2-24569

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Associated Pathways

MAPK Signaling: Oxidative Stress Pathway MAPK Signaling: Oxidative Stress Pathway Thumbnail