beta-Catenin Antibody - IHC-Prediluted

Name: beta-Catenin Antibody - IHC-Prediluted
Brand: Novus Biologicals
SKU: NBP2-48367
Price: 449 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-48367

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

IHC-Prediluted

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Product Specifications

Immunogen

A synthetic peptide from the middle of beta-Catenin protein (Uniprot: P35222)

Reactivity Notes

The amino acid sequence used as the immunogen for this beta Catenin antibody is 100% homologous in a broad range of species.

Localization

Cell surface and cytoplasmic

Marker

Epithelial Cell Marker, Adherens Junction Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Scientific Data Images for beta-Catenin Antibody - IHC-Prediluted

Immunohistochemistry-Paraffin: beta-Catenin Antibody - IHC-Prediluted [NBP2-48367]

Immunohistochemistry-Paraffin: beta-Catenin Antibody - IHC-Prediluted [NBP2-48367] - Formalin-fixed, paraffin-embedded breast lobular carcinoma stained with Catenin, beta PAb. Note cytoplasmic staining in lobular carcinoma.

Immunohistochemistry-Paraffin: beta-Catenin Antibody - IHC-Prediluted [NBP2-48367]

Immunohistochemistry-Paraffin: beta-Catenin Antibody - IHC-Prediluted [NBP2-48367] - Formalin-fixed, paraffin-embedded breast ductal carcinoma stained with Catenin, beta PAb. Note membrane staining in ductal carcinoma.

Western Blot: beta-Catenin Antibody - IHC-Prediluted [NBP2-48367] -

Western Blot of Catenin, beta (p120) in human brain 1) absence and 2) presence of immunizing peptide and 3) Mouse brain lysate using beta-Catenin Antibody - IHC-Prediluted

Flow Cytometry: beta-Catenin Antibody - IHC-Prediluted [NBP2-48367] -

Flow Cytometric Analysis of PFA fixed HeLa cells using beta-Catenin Antibody - IHC-Prediluted followed by goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red).

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Applications for beta-Catenin Antibody - IHC-Prediluted

Application

Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

10 mM PBS with 0.05% BSA

Format

IHC-Prediluted

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: beta-Catenin

The protein encoded by the beta Catenin gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs arenecessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion betweencells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contactinhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this proteinbinds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this geneare a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Three transcript variants encoding the same protein have been found for this gene.

Alternate Names

bCatenin, CTNNB1

Gene Symbol

CTNNB1

Additional beta-Catenin Products

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Product Specific Notices for beta-Catenin Antibody - IHC-Prediluted

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.