beta-Catenin Antibody - BSA Free

Name: beta-Catenin Antibody - BSA Free
Brand: Novus Biologicals
SKU: NBP2-33124
Price: 479 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-33124

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

View all beta-Catenin Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide from the middle of beta-Catenin protein (Uniprot: P35222)

Reactivity Notes

The amino acid sequence used as the immunogen for this beta Catenin antibody is 100% homologous in a broad range of species.

Localization

Cell surface and cytoplasmic

Marker

Epithelial Cell Marker, Adherens Junction Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS WITHOUT BSA. Also available at 200 ug/ml WITH BSA & azide (NBP2-29476).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for beta-Catenin Antibody - BSA Free

Western Blot: beta-Catenin AntibodyBSA Free [NBP2-33124]

Western Blot: beta-Catenin Antibody - BSA Free [NBP2-33124] - Western Blot of beta-Catenin (p120) in human brain 1) absence and 2) presence of immunizing peptide and 3) Mouse brain lysate using beta-Catenin PAb.

Immunohistochemistry-Paraffin: beta-Catenin Antibody - BSA Free [NBP2-33124]

Immunohistochemistry-Paraffin: beta-Catenin Antibody - BSA Free [NBP2-33124] - Formalin-paraffin breast lobular carcinoma stained with catenin, beta PAb. Note cytoplasmic staining in lobular carcinoma.

Flow Cytometry: beta-Catenin Antibody - BSA Free [NBP2-33124]

Flow Cytometry: beta-Catenin Antibody - BSA Free [NBP2-33124] - Flow Cytometric Analysis of PFA fixed HeLa cells using beta-Catenin PAb followed by goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red).

Immunohistochemistry-Paraffin: beta-Catenin Antibody - BSA Free [NBP2-33124]

Immunohistochemistry-Paraffin: beta-Catenin Antibody - BSA Free [NBP2-33124] - Formalin-fixed, paraffin-embedded human breast ductal carcinoma stained with beta- Catenin antibody (1:100), peroxidase-conjugate and Warp Red chromogen (20X). Note specific membrane staining in ductal carcinoma.

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Applications for beta-Catenin Antibody - BSA Free

Application

Recommended Usage

Flow Cytometry

0.5-1ug/million cells

Immunohistochemistry

0.5-1ug/ml

Immunohistochemistry-Paraffin

0.5-1ug/ml

Western Blot

0.5-1ug/ml

Application Notes

Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

10 mM PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: beta-Catenin

The protein encoded by the beta Catenin gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs arenecessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion betweencells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contactinhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this proteinbinds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this geneare a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Three transcript variants encoding the same protein have been found for this gene.

Alternate Names

bCatenin, CTNNB1

Gene Symbol

CTNNB1

Additional beta-Catenin Products

Product Documents for beta-Catenin Antibody - BSA Free

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Certificate of Analysis

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Product Specific Notices for beta-Catenin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.