Beta Hydroxysteroid Dehydrogenase Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32353

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Beta Hydroxysteroid Dehydrogenase Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Beta Hydroxysteroid Dehydrogenase. The exact sequence is proprietary.

Reactivity Notes

Dog (80%), Cat (81%).

Localization

Endoplasmic reticulum membrane, Mitochondrion membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Beta Hydroxysteroid Dehydrogenase Antibody - BSA Free

Western Blot: Beta Hydroxysteroid Dehydrogenase Antibody [NBP1-32353]

Western Blot: Beta Hydroxysteroid Dehydrogenase Antibody [NBP1-32353]

Western Blot: Beta Hydroxysteroid Dehydrogenase Antibody [NBP1-32353] - Sample (30 ug of whole cell lysate)A:HeLa S3 B:MOLT4 12% SDS PAGE, antibody diluted at 1:500.
Immunohistochemistry-Paraffin: Beta Hydroxysteroid Dehydrogenase Antibody [NBP1-32353]

Immunohistochemistry-Paraffin: Beta Hydroxysteroid Dehydrogenase Antibody [NBP1-32353]

Immunohistochemistry-Paraffin: Beta Hydroxysteroid Dehydrogenase Antibody [NBP1-32353] - Human normal liver. HSD3B2 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Applications for Beta Hydroxysteroid Dehydrogenase Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Assay dependent

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Reviewed Applications

Read 1 review rated 3 using NBP1-32353 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Beta Hydroxysteroid Dehydrogenase

The protein encoded by the Beta Hydroxysteroid Dehydrogenases gene is a bifunctional enzyme that catalyzes the oxidative conversion ofdelta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. It plays a crucial role in thebiosynthesis of all classes of hormonal steroids. This gene is predominantly expressed in the adrenals and the gonads.Mutations in this gene are associated with 3-beta-hydroxysteroid dehydrogenase, type II, deficiency. Alternativelyspliced transcript variants have been found for this gene. (provided by RefSeq)

Alternate Names

3 beta-HSD type II, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II, 3-beta-HSD II, delta 5-delta 4-isomerase type II, HSD3B, HSDB, HSDB3B, hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2,3-beta-hydroxy-Delta(5)-steroid dehydrogenase, progesterone reductase, SDR11E2, short chain dehydrogenase/reductase family 11E, member 2,3-beta-hydroxy-5-ene steroid dehydrogenase

Gene Symbol

HSD3B2

Additional Beta Hydroxysteroid Dehydrogenase Products

Product Documents for Beta Hydroxysteroid Dehydrogenase Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Beta Hydroxysteroid Dehydrogenase Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for Beta Hydroxysteroid Dehydrogenase Antibody - BSA Free

Customer Reviews for Beta Hydroxysteroid Dehydrogenase Antibody - BSA Free (1)

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  • Beta Hydroxysteroid Dehydrogenase Antibody
    Name: Paula Aliberti
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Human testis
    Species: Human
    Verified Customer | Posted 10/09/2017
    Three month old human testis. HSD3B2 expression in matures Leydig cells at minipuberty as well as in some peritbular myoid cells (probably pre-Leydig cells).
    Primary Ab diluted 1/100. In most samples, a background coloring inside the seminiferous tubule was observed.
    Beta Hydroxysteroid Dehydrogenase Antibody - BSA Free NBP1-32353

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