beta I Tubulin Antibody (OTI4A3)

Western Blot: beta I Tubulin Antibody (OTI4A3) [NBP2-46245]

Western Blot: beta I Tubulin Antibody (OTI4A3) [NBP2-46245] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemistry: beta I Tubulin Antibody (OTI4A3) [NBP2-46245]

Immunohistochemistry: beta I Tubulin Antibody (OTI4A3) [NBP2-46245] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: beta I Tubulin Antibody (OTI4A3) [NBP2-46245]

Immunohistochemistry: beta I Tubulin Antibody (OTI4A3) [NBP2-46245] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: beta I Tubulin Antibody (OTI4A3) [NBP2-46245]

Immunohistochemistry: beta I Tubulin Antibody (OTI4A3) [NBP2-46245] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: beta I Tubulin Antibody (OTI4A3) [NBP2-46245]

Immunohistochemistry: beta I Tubulin Antibody (OTI4A3) [NBP2-46245] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Western Blot: beta I Tubulin Antibody (OTI4A3) [NBP2-46245] -

Microtubule growth and tubulin expression following cLTP induction. a Live cell imaging of DIV 11/12 cultured hippocampal neurons transfected with EB3-Tomato and exposed to chemical induction of LTP. To induce cLTP, neurons transfected at DIV8-9 were subjected to cLTP induction 3 days post transfection. cLTP was induced by treatment with 50 uM Forskolin, 100 nM Rolipram and 100 uM Picrotoxin for 10 min in Ringer solution without Mg2+, following 10 min of recovery in Ringer solution containing Mg2+. Control cells were kept in Ringer solution containing Mg2+. Image acquisition: 1 frame/s over 3 min. Scale bar, 20 um. EB3 comets in axonal segments in colored boxes of overview images (top panel) are visualized by kymographs (bottom panel). b Quantification of a, depicting median values (N = 3 experiments). ***p < 0.001. Each data point represents one EB3 comet; control (n = 54), cLTP (n = 70). For graphical representation of the distribution, data are binned with a bin size of 0.02 um/s. Statistics: Mann–Whitney Rank Sum Test. c–j Quantification of tubulin isotype expression in acute hippocampal slices, following chemical induction of LTP. Control slices were kept in ACSF. cLTP slices were incubated in ACSF with TEA for 10 min and further incubated in pure ACSF over 0, 10 or 20 min. (gamma-Adaptin: loading control; N = 3 experiments, each). Representative western blots and the corresponding quantification of (c, d) pan-Tuba, (e, f) pan-Tubb, (g, h) Tubb1 and (i, j) Tubb3. Depicted are median values. Black dots show the mean value. For graphical representation of the distribution, data are binned with a bin size of 0.05 a.u. Statistics: One way ANOVA followed by Student's t-test or Mann–Whitney Rank Sum Test. *p < 0.05, **p < 0.01 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36309617), licensed under a CC-BY license. Not internally tested by Novus Biologicals.