beta II Tubulin A Antibody (3B2) - Azide and BSA Free

Novus Biologicals | Catalog # H00007280-M04

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3B2

Format

Azide and BSA Free
View all beta II Tubulin A Primary Antibodies »

Product Specifications

Immunogen

TUBB2A (AAH01194, 1 a.a. ~ 445 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEAAGNKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETYSIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDSKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNTNDLVSEYQQYQDATADEQGEFEEEEGEDEA

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for beta II Tubulin A Antibody (3B2) - Azide and BSA Free

Western Blot: beta II Tubulin A Antibody (3B2) [H00007280-M04]

Western Blot: beta II Tubulin A Antibody (3B2) [H00007280-M04]

Western Blot: beta II Tubulin A Antibody (3B2) [H00007280-M04] - Analysis of TUBB2A expression in Jurkat (Cat # L017V1).
Immunocytochemistry/ Immunofluorescence: beta II Tubulin A Antibody (3B2) [H00007280-M04]

Immunocytochemistry/ Immunofluorescence: beta II Tubulin A Antibody (3B2) [H00007280-M04]

Immunocytochemistry/Immunofluorescence: beta II Tubulin A Antibody (3B2) [H00007280-M04] - Analysis of monoclonal antibody to TUBB2A on HeLa cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: beta II Tubulin A Antibody (3B2) [H00007280-M04]

Immunohistochemistry-Paraffin: beta II Tubulin A Antibody (3B2) [H00007280-M04]

Immunohistochemistry-Paraffin: beta II Tubulin A Antibody (3B2) [H00007280-M04] - Analysis of monoclonal antibody to TUBB2A on formalin-fixed paraffin-embedded human colon. Antibody concentration 1.5 ug/ml
Western Blot: beta II Tubulin A Antibody (3B2) [H00007280-M04]

Western Blot: beta II Tubulin A Antibody (3B2) [H00007280-M04]

Western Blot: beta II Tubulin A Antibody (3B2) [H00007280-M04] - Analysis of TUBB2A expression in PC-12 (Cat # L012V1).
Western Blot: beta II Tubulin A Antibody (3B2) [H00007280-M04]

Western Blot: beta II Tubulin A Antibody (3B2) [H00007280-M04]

Western Blot: beta II Tubulin A Antibody (3B2) [H00007280-M04] - Analysis of TUBB2A expression in Raw 264.7 (Cat # L024V1).
Western Blot: beta II Tubulin A Antibody (3B2) [H00007280-M04]

Western Blot: beta II Tubulin A Antibody (3B2) [H00007280-M04]

Western Blot: beta II Tubulin A Antibody (3B2) [H00007280-M04] - Analysis of TUBB2A expression in NIH/3T3 (Cat # L018V1).

Applications for beta II Tubulin A Antibody (3B2) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
It has been used for ELISA, WB and IF.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: beta II Tubulin A

Microtubules are essential parts in eukaryotic cell structures, transportation, and mitosis and consists mainly of 2 soluble protein subunits, alpha and beta tubulin. Beta-tubulin binds to alpha tubulin to form tubulin heterodimer which is post-translationally modified (1). The tubulin dimer complex binds to GTP and assembles onto the positive ends of microtubules. After incorporation into the microtubules, bound GTP is hydrolyzed by beta tubulin. The stability of the dimer in the microtubules is depended on presence of beta tubulin, where dimer with GTP bound beta-tubulin is stable to microtubule incorporation (2). Mutation on beta-tubulin has been linked to anti-cancer drug resistance in lung cancers (3).

Long Name

Tubulin beta-2A chain

Alternate Names

TUBB2, TUBB2A

Gene Symbol

TUBB2A

UniProt

AAH01194 (Human)

Additional beta II Tubulin A Products

Product Documents for beta II Tubulin A Antibody (3B2) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for beta II Tubulin A Antibody (3B2) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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