beta Sarcoglycan Antibody (1E3) - Azide and BSA Free

Novus Biologicals | Catalog # H00006443-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1E3

Format

Azide and BSA Free
View all beta-Sarcoglycan Primary Antibodies »

Product Specifications

Immunogen

SGCB (AAH20709, 1 a.a. ~ 318 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAAAAAAAAEQQSSNGPVKKSMREKAVERRSVNKEHNSNFKAGYIPIDEDRLHKTGLRGRKGNLAICVIILLFILAVINLIITLVIWAVIRIGPNGCDSMEFHESGLLRFKQVSDMGVIHPLYKSTVGGRRNENLVITGNNQPIVFQQGTTKLSVENNKTSITSDIGMQFFDPRTQNILFSTDYETHEFHLPSGVKSLNVQKASTERITSNATSDLNIKVDGRAIVRGNEGVFIMGKTIEFHMGGNMELKAENSIILNGSVMVSTTRLPSSSSGDQLGSGDWVRYKLCMCADGTLFKVQVTSQNMGCQISDNPCGNTH

Specificity

SGCB - sarcoglycan, beta (43kDa dystrophin-associated glycoprotein)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for beta Sarcoglycan Antibody (1E3) - Azide and BSA Free

Immunohistochemistry-Paraffin: beta Sarcoglycan Antibody (1E3) [H00006443-M01]

Immunohistochemistry-Paraffin: beta Sarcoglycan Antibody (1E3) [H00006443-M01]

Immunohistochemistry-Paraffin: beta Sarcoglycan Antibody (1E3) [H00006443-M01] - Analysis of monoclonal antibody to SGCB on formalin-fixed paraffin-embedded human heart. Antibody concentration 3 ug/ml.
Sandwich ELISA: beta Sarcoglycan Antibody (1E3) [H00006443-M01] - Detection limit for recombinant GST tagged SGCB is approximately 0.3ng/ml as a capture antibody.

Applications for beta Sarcoglycan Antibody (1E3) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Sandwich ELISA

1:100-1:2000

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: beta-Sarcoglycan

The dystrophin-glycoprotein complex (DGC) is a multisubunit protein complex that spans the sarcolemma and provides structural linkage between the subsarcolemmal cytoskeleton and the extracellular matrix of muscle cells. There are 3 main subcomplexes of the DGC: the cytoplasmic proteins dystrophin (DMD; MIM 300377) and syntrophin (SNTA1; MIM 601017), the alpha- and beta-dystroglycans (see MIM 128239), and the sarcoglycans (see, e.g., SGCA; MIM 600119) (Crosbie et al., 2000 [PubMed 10942431]).[supplied by OMIM]

Alternate Names

43DAG, A3b, beta Sarcoglycan, Beta-SG, Sarcoglycan beta, SGC, SGCB

Entrez Gene IDs

6443 (Human)

Gene Symbol

SGCB

OMIM

600900 (Human)

UniProt

AAH20709 (Human)

Additional beta-Sarcoglycan Products

Product Documents for beta Sarcoglycan Antibody (1E3) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for beta Sarcoglycan Antibody (1E3) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cardiac Stem Cell Markers
Proteoglycans

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Protocols

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FAQs

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