beta-TrCP1/BTRC Antibody (4C5D8) - BSA Free

Novus Biologicals | Catalog # NBP2-61712

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4C5D8

Format

BSA Free
View all beta-TrCP1/BTRC Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human beta-TrCP1/BTRC (AA: 24-151) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

68.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for beta-TrCP1/BTRC Antibody (4C5D8) - BSA Free

Western Blot: beta-TrCP1/BTRC Antibody (4C5D8)BSA Free [NBP2-61712]

Western Blot: beta-TrCP1/BTRC Antibody (4C5D8)BSA Free [NBP2-61712]

Western Blot: beta-TrCP1/BTRC Antibody (4C5D8) [NBP2-61712] - Analysis using BTRC mAb against HEK293 (1) and BTRC (AA: 24-151)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry: beta-TrCP1/BTRC Antibody (4C5D8) - BSA Free [NBP2-61712]

Immunohistochemistry: beta-TrCP1/BTRC Antibody (4C5D8) - BSA Free [NBP2-61712]

Immunohistochemistry: beta-TrCP1/BTRC Antibody (4C5D8) [NBP2-61712] - Analysis of paraffin-embedded rectum cancer tissues using BTRC mouse mAb with DAB staining.
Flow Cytometry: beta-TrCP1/BTRC Antibody (4C5D8) - BSA Free [NBP2-61712]

Flow Cytometry: beta-TrCP1/BTRC Antibody (4C5D8) - BSA Free [NBP2-61712]

Flow Cytometry: beta-TrCP1/BTRC Antibody (4C5D8) [NBP2-61712] - Analysis of Hela cells using BTRC mouse mAb (green) and negative control (red).
Western Blot: beta-TrCP1/BTRC Antibody (4C5D8)BSA Free [NBP2-61712]

Western Blot: beta-TrCP1/BTRC Antibody (4C5D8)BSA Free [NBP2-61712]

Western Blot: beta-TrCP1/BTRC Antibody (4C5D8) [NBP2-61712] - Analysis using BTRC mAb against human BTRC (AA: 24-151) recombinant protein. (Expected MW is 40.2 kDa)
Western Blot: beta-TrCP1/BTRC Antibody (4C5D8)BSA Free [NBP2-61712]

Western Blot: beta-TrCP1/BTRC Antibody (4C5D8)BSA Free [NBP2-61712]

Western Blot: beta-TrCP1/BTRC Antibody (4C5D8) [NBP2-61712] - Analysis using BTRC mouse mAb against Ramos (1), MCF-7 (2), and K562 (3) cell lysate.
Immunohistochemistry: beta-TrCP1/BTRC Antibody (4C5D8) - BSA Free [NBP2-61712]

Immunohistochemistry: beta-TrCP1/BTRC Antibody (4C5D8) - BSA Free [NBP2-61712]

Immunohistochemistry: beta-TrCP1/BTRC Antibody (4C5D8) [NBP2-61712] - Analysis of paraffin-embedded esophageal cancer tissues using BTRC mouse mAb with DAB staining.
ELISA: beta-TrCP1/BTRC Antibody (4C5D8) - BSA Free [NBP2-61712]

ELISA: beta-TrCP1/BTRC Antibody (4C5D8) - BSA Free [NBP2-61712]

ELISA: beta-TrCP1/BTRC Antibody (4C5D8) [NBP2-61712] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Applications for beta-TrCP1/BTRC Antibody (4C5D8) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:100-1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: beta-TrCP1/BTRC

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class; in addition to an F-box, this protein contains multiple WD-40 repeats. The encoded protein mediates degradation of CD4 via its interaction with HIV-1 Vpu. It has also been shown to ubiquitinate phosphorylated NFKBIA (nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha), targeting it for degradation and thus activating nuclear factor kappa-B. Alternatively spliced transcript variants have been described. A related pseudogene exists in chromosome 6.

Long Name

Beta-Transducin Repeat Containing E3 Ubiquitin Protein Ligase

Alternate Names

betaTrCP1, BTRC, BTRCP, E3RSIkappaB, FBW1A, FBXW1A, FWD1

Gene Symbol

BTRC

Additional beta-TrCP1/BTRC Products

Product Documents for beta-TrCP1/BTRC Antibody (4C5D8) - BSA Free

Certificate of Analysis

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Product Specific Notices for beta-TrCP1/BTRC Antibody (4C5D8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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