beta Tubulin Antibody (4E9G8)

Novus Biologicals | Catalog # NBP3-15439

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4E9G8 expressed in HEK293
View all beta Tubulin Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 250-350 of human beta Tubulin (P07437). LRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQVFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVK

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for beta Tubulin Antibody (4E9G8)

Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody (4E9G8) [NBP3-15439]

Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody (4E9G8) [NBP3-15439]

Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody (4E9G8) [NBP3-15439] - Immunofluorescence analysis of C6 cells using beta Tubulin antibody (NBP3-15439) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody (4E9G8) [NBP3-15439]

Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody (4E9G8) [NBP3-15439]

Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody (4E9G8) [NBP3-15439] - Immunofluorescence analysis of NIH/3T3 cells using beta Tubulin antibody (NBP3-15439) at dilution of 1:100. Blue: DAPI for nuclear staining.
beta Tubulin Antibody (4E9G8)

Western Blot: beta Tubulin Antibody (4E9G8) [NBP3-15439] -

Western Blot: beta Tubulin Antibody (4E9G8) [NBP3-15439] - Analysis of various lysates using beta -Tubulin Rabbit mAb at 1:5000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 20s.
beta Tubulin Antibody (4E9G8)

Western Blot: beta Tubulin Antibody (4E9G8) [NBP3-15439] -

Western Blot: beta Tubulin Antibody (4E9G8) [NBP3-15439] - Analysis of lysates from 293F cells, using beta -Tubulin Rabbit mAb at 1:1000-1:30000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.
beta Tubulin Antibody (4E9G8)

Immunohistochemistry-Paraffin: beta Tubulin Antibody (4E9G8) [NBP3-15439] -

Immunohistochemistry-Paraffin: beta Tubulin Antibody (4E9G8) [NBP3-15439] -Analysis of paraffin-embedded Rat kidney tissue using beta -Tubulin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
beta Tubulin Antibody (4E9G8)

Western Blot: beta Tubulin Antibody (4E9G8) [beta Tubulin] -

Western Blot: beta Tubulin Antibody (4E9G8) [beta Tubulin] - Western blot analysis of various lysates using beta Tubulin Rabbit mAb at 1:5000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 20s.
beta Tubulin Antibody (4E9G8)

Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody (4E9G8) [beta Tubulin] -

Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody (4E9G8) [beta Tubulin] - Immunofluorescence analysis of U2OS cells using beta Tubulin Rabbit mAb at dilution of 1:100. Blue: DAPI for nuclear staining.
beta Tubulin Antibody (4E9G8)

Immunoprecipitation: beta Tubulin Antibody (4E9G8) [beta Tubulin] -

Immunoprecipitation: beta Tubulin Antibody (4E9G8) [beta Tubulin] - Immunoprecipitation analysis of 300 ug extracts of 293T cells using 3 ug beta Tubulin antibody. Western blot was performed from the immunoprecipitate using beta Tubulin antibody at a dilution of 1:1000.

Applications for beta Tubulin Antibody (4E9G8)

Application
Recommended Usage

ELISA

,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Immunoprecipitation

0.5μg-4μg antibody for 200μg-400μg extracts of whole cells

Western Blot

1:5000 - 1:10000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: beta Tubulin

Beta Tubulin antibodies are useful as loading controls for Western Blotting and ELISA, as well as in studies invlolving cell structure and division. Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton.

Long Name

Tubulin beta chain

Alternate Names

OK/SW-cl.56, TUBB, TUBB5

Gene Symbol

TUBB

Additional beta Tubulin Products

Product Documents for beta Tubulin Antibody (4E9G8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for beta Tubulin Antibody (4E9G8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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