beta Tubulin Antibody (S11B) - Chimeric - Azide and BSA Free

Western Blot: beta Tubulin Antibody (S11B)ChimericAzide and BSA Free [NBP2-81064]

Western Blot: beta Tubulin Antibody (S11B) [NBP2-81064] - Western Blot using anti-Beta-Tubulin antibody (S11B) [NBP2-81064]. HeLa (A), A431 (B), HEK293 (C) and MCF-7 (D) cell lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of S11B [NBP2-81064] at 0.01 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Beta-Tubulin is ~54kDa. NBP2-81064 successfully detected human Beta-Tubulin in HeLa, A431, HEK293 and MCF-7 cell lysate samples.

Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody (S11B) - Chimeric - Azide and BSA Free [NBP2-81064]

Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody (S11B) [NBP2-81064] - Immunofluoresence staining of fixed HeLa cells with anti-Beta-tubulin antibody (S11B) [NBP2-81064]. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of S11B [NBP2-81064] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81064, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.

Immunohistochemistry-Paraffin: beta Tubulin Antibody (S11B) - Chimeric - Azide and BSA Free [NBP2-81064]

Immunohistochemistry-Paraffin: beta Tubulin Antibody (S11B) [NBP2-81064] - Immunohistochemical staining of human cerebral cortex tissue using [NBP2-81064]. Anti-Beta Tubulin staining of paraffin embedded human cerebral cortex tissue using the rabbit-chimeric version of S11B [NBP2-81064]. Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-81064 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Staining of neuronal cell bodies and their processes may be observed. Recommended concentration, 2-4 ug/ml.