Bim Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-77118

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Bim Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to amino acids near the center of human Bim. The sequence is identical to that of mouse and rat. The immunogen is located within amino acids 80 - 130 of Bim. Amino Acid Squence: SGYFSFDTDRSPAPMS

Specificity

Human BIM has 3 isoforms, including isoform EL (198aa, 22kD), isoform L (138aa, 16kD) and isoform S (108aa, 13kD). Mouse BIM has 3 isoforms, including isoform EL (196aa, 22kD), isoform L (140aa, 16kD) and isoform S (110aa, 13kD). Rat BIM has 4 isoforms, including isoform BOD-L (196aa, 22kD), isoform L (140aa, 16kD) and isoform BOD-M (110aa, 13kD). NBP1-77118 only detects isoform EL.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Bim Antibody - BSA Free

Western Blot: Bim AntibodyBSA Free [NBP1-77118]

Western Blot: Bim AntibodyBSA Free [NBP1-77118]

Western Blot: Bim Antibody [NBP1-77118] - Western Blot Validation in Human TissueLoading: 15 ug of lysates per lane.Antibodies: BIM NBP1-77118, (5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: Human urinary bladderLane 2: Human pancreas
Western Blot: Bim AntibodyBSA Free [NBP1-77118]

Western Blot: Bim AntibodyBSA Free [NBP1-77118]

Western Blot: Bim Antibody [NBP1-77118] - IAV via Protein Expression Profile in Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: BIM NBP1-76963 (0.5 ug/ml), BIM NBP1-77118 (5 ug/ml), beta-actin (1 ug/ml) and GAPDH (0.02 ug/ml), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Bim Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Bim Antibody - BSA Free [NBP1-77118] -

Immunocytochemistry/ Immunofluorescence: Bim Antibody - BSA Free [NBP1-77118] - Validation of BIM in K562 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling BIM with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
Bim Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Bim Antibody - BSA Free [NBP1-77118] -

Immunocytochemistry/ Immunofluorescence: Bim Antibody - BSA Free [NBP1-77118] - Validation of BIM K562 Cells. Immunohistochemical analysis of K562 cells using anti-BIM antibody at 10 ug/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Bim Antibody - BSA Free

Western Blot: Bim Antibody - BSA Free [NBP1-77118] -

Western Blot: Bim Antibody - BSA Free [NBP1-77118] - Validation in Human Cell Lines. Loading: 15 ug of lysates per lane.Antibodies: BIM, (5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Applications for Bim Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

10-20 ug/mL

Western Blot

5 ug/mL

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Bim

Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bim or BOD in human, mouse and rat. Bim/BOD interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. Bim/BOD induces apoptosis. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines.

Alternate Names

BAM, bcl-2 interacting mediator of cell death, bcl-2 interacting protein Bim, Bcl2-interacting mediator of cell death, Bcl2-L-11, BCL2-like 11 (apoptosis facilitator), bcl-2-like protein 11, bcl-2-related ovarian death agonist, BIM-alpha6, BIM-beta6, BIM-beta7, BIMBOD, BimEL, BimL

Gene Symbol

BCL2L11

UniProt

O43521 (Human)

Additional Bim Products

Product Documents for Bim Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Bim Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Bim Antibody - BSA Free

Showing  1 - 2 of 2 FAQs Showing All

  • Q: Please recommend which isotype antibody I should use with the NBP1-75511AF647 (Bim-AF647.) The product datasheet only says IgG, but no subclass or product number.

    A: This item is a mouse IgG1 antibody. For isotype control, I would recommend NBP1-43319AF647 or NBP2-27287AF647.

  • Q: Would you recommend a standard Fix/Perm buffer for Bim staining?

    A:

    Our lab used our standard ICC protocol for cell staining with this product. We used tween-20 in the permeabilization step as BIM can be found in the endomembrane system and mitochondria.

  • Q: Please recommend which isotype antibody I should use with the NBP1-75511AF647 (Bim-AF647.) The product datasheet only says IgG, but no subclass or product number.

    A: This item is a mouse IgG1 antibody. For isotype control, I would recommend NBP1-43319AF647 or NBP2-27287AF647.

  • Q: Would you recommend a standard Fix/Perm buffer for Bim staining?

    A:

    Our lab used our standard ICC protocol for cell staining with this product. We used tween-20 in the permeabilization step as BIM can be found in the endomembrane system and mitochondria.

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