BIN1 Antibody (99D) - BSA Free

Novus Biologicals | Catalog # NBP2-21673

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 99D

Format

BSA Free
View all BIN1 Primary Antibodies »

Product Specifications

Immunogen

BIN1 Antibody (99D) was produced in mouse by repeated immunizations with a fragment portion of recombinant human BIN1 protein followed by hybridoma development. (Uniprot: O00499)

Reactivity Notes

A BLAST analysis was used to suggest cross-reactivity with BIN1 from human and mouse sources based on 100% homology with the immunizing sequence. Cross-reactivity with BIN1 from other sources has not been determined.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

This antibody was purified from clarified mouse ascetic fluid by Protein A chromatography followed by extensive dialysis against the buffer stated above

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for BIN1 Antibody (99D) - BSA Free

Western Blot: BIN1 Antibody (99D) [NBP2-21673]

Western Blot: BIN1 Antibody (99D) [NBP2-21673]

Western Blot: BIN1 Antibody (99D) [NBP2-21673] - Lane 1: Keratinocyte derived from Bin1 wild type mice. Lane 2: Keratinocyte derived from Bin1 null mice. Load: 35 ug per lane. BIN1 monoclonal Antibody. Secondary antibody: Dye800 mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 1xPBS, 0.4% Tween-20. Other band: non-specific.
BIN1 Antibody (99D)

BIN1 Antibody (99D)

Flow Cytometry of Mouse Anti-BIN1 Antibody. Cells: C2C12 cells. Stimulation: none. Primary antibody: Anti-IgD (control), Antibody (99D clone). Secondary antibody: Biotin mouse secondary antibody at 1:10,000 for 45 min at RT and streptavidin PE at 1:5,000 for 30 min at RT.

Applications for BIN1 Antibody (99D) - BSA Free

Application
Recommended Usage

ELISA

1:5000-1:50000

Flow Cytometry

0.5-1x10^6 cells

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

10-100 ul

Western Blot

1:500-1:1500
Application Notes
This product has been tested for use in ELISA, IP, and Western Blot. This antibody is suitable for use in IHC and Flow Cytometry. Specific conditions for reactivity should be optimized by the end user.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: BIN1

BIN1 encodes several isoforms of a nucleocytoplasmic adaptor protein, one of which was initially identified as a MYC-interacting protein with features of a tumor suppressor. Isoforms that are expressed in the central nervous system may be involved in synaptic vesicle endocytosis and may interact with dynanim, synaptojanin, endophilin, and clathrin. Isoforms that are expressed in muscle and ubiquitously expressed isoforms localize to the cytoplasm and nucleus and activate a caspase independent apoptotic process. Studies in mouse suggest that this gene plays an important role in cardiac muscle development. Alternate splicing of the gene results in ten transcript variants encoding different isoforms. Aberrant splice variants expressed in tumor cell lines have also been described.

Alternate Names

AMPH2, Amphiphysin II, Amphiphysin-like protein, AMPHLDKFZp547F068, box dependant MYC interacting protein 1, bridging integrator 1Box-dependent myc-interacting protein 1, MGC10367, myc box-dependent-interacting protein 1, SH3P9

Gene Symbol

BIN1

UniProt

O00499 (Human)

Additional BIN1 Products

Product Documents for BIN1 Antibody (99D) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for BIN1 Antibody (99D) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for BIN1 Antibody (99D) - BSA Free

There are currently no reviews for this product. Be the first to review BIN1 Antibody (99D) - BSA Free and earn rewards!

Have you used BIN1 Antibody (99D) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies