Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Dot Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # 99F

Format

BSA Free
View all BIN1 Primary Antibodies »

Product Specifications

Immunogen

BIN1 Antibody (99F) was produced in mouse by repeated immunizations with BIN1 polypeptide followed by hybridoma development. (Uniprot: O00499)

Reactivity Notes

Cross-reactivity with BIN1 from other sources has not been determined

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Description

This antibody was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for BIN1 Antibody (99F) - BSA Free

Immunocytochemistry/ Immunofluorescence: BIN1 Antibody (99F) [NBP2-21674]

Immunocytochemistry/ Immunofluorescence: BIN1 Antibody (99F) [NBP2-21674]

Immunocytochemistry/Immunofluorescence: BIN1 Antibody (99F) [NBP2-21674] - Cells: C2C12 cells during growth or differentiation. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: BIN-1 (Exon 10 specific, 99F) monoclonal antibody. Secondary antibody: mouse secondary antibody at 1:10,000 for 45 min at RT. Localization: BIN1 is nuclear and cytoplasmic. Staining: BIN 1 as green fluorescent signal.
BIN1 Antibody (99F)

BIN1 Antibody (99F)

Immunoprecipitation of Mouse anti-BIN1 antibody. Immunoprecipitates were separated by SDS-PAGE and visualized by fluorography. The differentiation-associated (Exon 10+) form of Bin1 is recognized by using Bin1 (Exon 10 specific, 99F) monoclonal antibody. Lane 1: BIN1 [35ug]. Primary antibody: Anti-BIN1 (Exon 10 specific, 99F) monoclonal antibody at 1:400 for overnight at 2-8C. Secondary antibody: IRDye800(TM) mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted MW: 64.7 kDa. Observed MW: ~65 kDa for BIN-1.
BIN1 Antibody (99F)

BIN1 Antibody (99F)

Western Blot of Mouse Anti-BIN1 antibody. Lane 1: C2C12 during growth. Lane 2: C2C12 during differentiation. Load: 35 ug per lane. Primary antibody: BIN1 antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 64.7 kDa, ~55 kDa for BIN-1. Other band(s): non-specifics.

Applications for BIN1 Antibody (99F) - BSA Free

Application
Recommended Usage

ELISA

1:5000-1:50000

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunoprecipitation

10-100 ul

Western Blot

1:500-1:1500
Application Notes
This product has been tested for use in ELISA, Western Blot, IP, and IF. Specific conditions for reactivity should be optimized by the end user.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: BIN1

BIN1 encodes several isoforms of a nucleocytoplasmic adaptor protein, one of which was initially identified as a MYC-interacting protein with features of a tumor suppressor. Isoforms that are expressed in the central nervous system may be involved in synaptic vesicle endocytosis and may interact with dynanim, synaptojanin, endophilin, and clathrin. Isoforms that are expressed in muscle and ubiquitously expressed isoforms localize to the cytoplasm and nucleus and activate a caspase independent apoptotic process. Studies in mouse suggest that this gene plays an important role in cardiac muscle development. Alternate splicing of the gene results in ten transcript variants encoding different isoforms. Aberrant splice variants expressed in tumor cell lines have also been described.

Alternate Names

AMPH2, Amphiphysin II, Amphiphysin-like protein, AMPHLDKFZp547F068, box dependant MYC interacting protein 1, bridging integrator 1Box-dependent myc-interacting protein 1, MGC10367, myc box-dependent-interacting protein 1, SH3P9

Gene Symbol

BIN1

UniProt

O00499 (Human)

Additional BIN1 Products

Product Documents for BIN1 Antibody (99F) - BSA Free

Certificate of Analysis

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Product Specific Notices for BIN1 Antibody (99F) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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