Key Product Details

Species Reactivity

Non-species specific

Applications

Knockout Validated, Immunocytochemistry/ Immunofluorescence

Label

Rhodamine (Excitation = 570 nm, Emission = 590 nm)

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH)

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room

Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA)-Immunoglobulin and Protease free
Color: Red Excitation Wavelength: 550 Emission Wavelength: 570
Conjugation Chemistry: Isothiocyanate

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and Biotin conjugated IgG

Applications for Biotin Antibody [Rhodamine] - F(ab')2

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500-1:2500
Application Notes
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Biotin

Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.

Long Name

Biotin

Alternate Names

Vitamin B4

Additional Biotin Products

Product Documents for Biotin Antibody [Rhodamine] - F(ab')2

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Biotin Antibody [Rhodamine] - F(ab')2

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for Biotin Antibody [Rhodamine] - F(ab')2

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  • Q: How large is Biotin label?

    A: Biotin is 244 Daltons (244.31 g/mol). This small size means the biological activity of a labled protein will most likely be unaffected by the addition of biotin.

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