Key Product Details

Species Reactivity

Non-species specific

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Fluorophore-linked immunosorbent assay

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Format

BSA Free
View all Biotin Primary Antibodies »

Product Specifications

Immunogen

Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH)

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Biotin conjugated IgG and Biotin conjugated Bovine Serum Albumin.

This product is stable for several weeks at 4C as an undiluted liquid. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Scientific Data Images for Biotin Antibody - BSA Free

Western Blot: Biotin Antibody [NB120-6643]

Western Blot: Biotin Antibody [NB120-6643]

Western Blot: Biotin Antibody [NB120-6643] - Analysis using the Texas Red conjugate of NB120-6643. Detection of Lane 1: Biotin conjugated Guinea Pig IgG. Lane 2: None. Load: 50 ng per lane.
Western Blot: Biotin Antibody [NB120-6643]

Western Blot: Biotin Antibody [NB120-6643]

Western Blot: Biotin Antibody [NB120-6643] - Analysis using the Fluorescein conjugate of NB120-6643. Detection of Lane 1: Biotin conjugated Guinea Pig IgG. Lane 2: None. Load: 50 ng per lane.
ELISA: Biotin Antibody [NB120-6643]

ELISA: Biotin Antibody [NB120-6643]

ELISA: Biotin Antibody [NB120-6643] - Analysis using BIOTIN Antibody DyLight 488.
ELISA: Biotin Antibody [NB120-6643]

ELISA: Biotin Antibody [NB120-6643]

ELISA: Biotin Antibody [NB120-6643] - ELISA results of purified Goat anti-Biotin Antibody Peroxidase Conjugated tested against purified biotin. Each well was coated in duplicate with 1.0 ug of Bovine Albumin Biotin Conjugated. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3 percent fish gelatin as blocking buffer and TMB substrate. Image using the HRP form of this antibody.

Applications for Biotin Antibody - BSA Free

Application
Recommended Usage

ELISA

1:20000

Immunohistochemistry

1:1000-1:5000

Immunohistochemistry-Paraffin

1:10 - 1:500

Western Blot

1:2000-1:10000
Application Notes
This product has been tested by ELISA and SDS-PAGE and is suitable for immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. Optimal concentrations in other immunoassays should be determined by the researcher.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Biotin

Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.

Long Name

Biotin

Alternate Names

Vitamin B4

Additional Biotin Products

Product Documents for Biotin Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Biotin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Biotin Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Biotin Antibody - BSA Free

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  • Q: How large is Biotin label?

    A: Biotin is 244 Daltons (244.31 g/mol). This small size means the biological activity of a labled protein will most likely be unaffected by the addition of biotin.

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