Key Product Details

Species Reactivity

Non-species specific

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Dot Blot

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH)

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA)-Immunoglobulin and Protease free
Color: Green Excitation Wavelength: 495 Emission Wavelength: 528
Conjugation Chemistry: Isothiocyanate

Fab fragment anti-Biotin Antibody [FITC]was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads followed by papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Fluorescein

Scientific Data Images for Biotin Antibody [FITC] - F(ab)

Biotin Antibody [FITC] - F(ab)

Biotin Antibody [FITC] - F(ab)

Dot Blot results of Goat Biotin Antibody [FITC] - F(ab). Dots top row: Biotin-BSA Conjugate. Dots bottom row: BSA alone. Load: (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: none. Secondary Antibody: Goat Fab Anti-Biotin Antibody FITC at 1ug/mL in 1hr RT. Imaged with BioRad ChemiDoc, Fluorescein filter.

Applications for Biotin Antibody [FITC] - F(ab)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500-1:2500

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:20-1:50

Western Blot

1:100-1:500
Application Notes
This product has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Specific conditions for reactivity should be optimized by the end user.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

Reconstitute with 1.0 ml deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Biotin

Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.

Long Name

Biotin

Alternate Names

Vitamin B4

Additional Biotin Products

Product Documents for Biotin Antibody [FITC] - F(ab)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Biotin Antibody [FITC] - F(ab)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Biotin Antibody [FITC] - F(ab)

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  • Q: How large is Biotin label?

    A: Biotin is 244 Daltons (244.31 g/mol). This small size means the biological activity of a labled protein will most likely be unaffected by the addition of biotin.

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