Catalog Number: 1760
Chemical Name: (±)-1,2,3,3a-Tetrahydro-3a-hydroxy-6-methyl-1-phenyl-4H-pyrrolo[2,3-b]quinolin-4-one
Biological Activity
Selective inhibitor of myosin II ATPase activity; inhibits contraction of the cleavage furrow without disrupting mitosis or contractile ring assembly. Rapidly and reversibly blocks cell blebbing, and disrupts directed cell migration and cytokinesis in vertebrate cells.
Technical Data
  • M.Wt:
  • Formula:
  • Solubility:
    Soluble to 100 mM in DMSO
  • Purity:
  • Storage:
    Store at +4°C
  • CAS No:
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis. All Tocris products are intended for laboratory research use only.
Additional Information
Other Product-Specific Information:
Background References
  1. Specificity of blebbistatin, an inhibitor of myosin II.
    Limouze et al.
    J.Muscle Res.Cell Motil., 2004;25:337
  2. Dissecting temporal and spatial control of cytokinesis with a myosin II inhibitor.
    Straight et al.
    Science, 2003;299:1743
  3. Non-muscle myosin II regulates survival threshold of pluripotent stem cells.
    Walker et al.
    Nat.Commun., 2010;1:71
  4. Dissecting cytokinesis with small molecules.
    Straight et al.
    Amer.Soc.Cell Biol.Abstr., 2002;:2515

The citations listed below are publications that use Tocris products. Selected citations for (±)-Blebbistatin include:

Showing Results 1 - 10 of 27

  1. MYH9 is an Essential Factor for Porcine Reproductive and Respiratory Syndrome Virus Infection.
    Authors: Gao Et al.
    Nat Commun
  2. Substrate stress relaxation regulates cell spreading.
    Authors: Chaudhuri Et al.
    Int J Nanomedicine
  3. Expression of α-Smooth Muscle Actin Determines the Fate of Mesenchymal Stromal Cells.
    Authors: Talele Et al.
    Stem Cell Reports
  4. Mechanical coupling between transsynaptic N-cadherin adhesions and actin flow stabilizes dendritic spines.
    Authors: Chazeau Et al.
    Int J Cancer
  5. ZO-1 controls endothelial adherens junctions, cell-cell tension, angiogenesis, and barrier formation.
    Authors: Tornavaca Et al.
  6. Cortical contractility triggers a stochastic switch to fast amoeboid cell motility.
    Authors: Ruprecht Et al.
    PLoS One
  7. SDF-1α stiffens myeloma bone marrow mesenchymal stromal cells through the activation of RhoA-ROCK-Myosin II.
    Authors: Choi Et al.
    J Cell Biol
  8. Atherosclerosis exacerbates arrhythmia following myocardial infarction: Role of myocardial inflammation.
    Authors: Jesus Et al.
    Mol Biol Cell
  9. Role of sarcoplasmic reticulum calcium in development of secondary calcium rise and early afterdepolarizations in long QT syndrome rabbit model.
    Authors: Chang Et al.
    J Cell Sci
  10. Simvastatin enhances Rho/actin/cell rigidity pathway contributing to mesenchymal stem cells' osteogenic differentiation.
    Authors: Tai Et al.
    Heart Rhythm
  11. Live Cell Interferometry Quantifies Dynamics of Biomass Partitioning during Cytokinesis.
    Authors: Zangle Et al.
    PLoS One
  12. Organ explant culture of neonatal rat ventricles: a new model to study gene and cell therapy.
    Authors: Haan Et al.
    PLoS One
  13. Sinoatrial node reentry in a canine chronic left ventricular infarct model: role of intranodal fibrosis and heterogeneity of refractoriness.
    Authors: Glukhov Et al.
    Circ Arrhythm Electrophysiol
  14. Mitochondrial dysfunction causing cardiac sodium channel downregulation in cardiomyopathy.
    Authors: Liu Et al.
    J Mol Cell Cardiol
  15. LTP induction translocates cortactin at distant synapses in wild-type but not Fmr1 knock-out mice.
    Authors: Seese Et al.
    J Neurosci
  16. A Rho GTPase signal treadmill backs a contractile array.
    Authors: Burkel Et al.
    Dev Cell
  17. Contractile Activity Regulates Inducible Nitric Oxide Synthase Expression and NO(i) Production in Cardiomyocytes via a FAK-Dependent Signaling Pathway.
    Authors: Chu Et al.
    J Signal Transduct
  18. Optical mapping of action potentials and calcium transients in the mouse heart.
    Authors: Lang Et al.
    J Vis Exp
  19. Effects of KATP channel openers diazoxide and pinacidil in coronary-perfused atria and ventricles from failing and non-failing human hearts.
    Authors: Fedorov Et al.
    J Mol Cell Cardiol
  20. RhoJ/TCL regulates endothelial motility and tube formation and modulates actomyosin contractility and focal adhesion numbers.
    Authors: Kaur Et al.
    Arterioscler Thromb Vasc Biol
  21. Role of Pyk2 in cardiac arrhythmogenesis.
    Authors: Lang Et al.
    J Inflamm (Lond)
  22. 'White wave' analysis of epithelial scratch wound healing reveals how cells mobilise back from the leading edge in a myosin-II-dependent fashion.
    Authors: Matsubayashi Et al.
    Proc Natl Acad Sci U S A
  23. Transmural heterogeneity and remodeling of ventricular excitation-contraction coupling in human heart failure.
    Authors: Lou Et al.
  24. Emergence of patterned stem cell differentiation within multicellular structures.
    Authors: Ruiz and Chen
    Sci Rep
  25. Rac1-null mouse embryonic fibroblasts are motile and respond to platelet-derived growth factor.
    Authors: Vidali Et al.
    Mol Biol Cell
  26. Reassembly of contractile actin cortex in cell blebs.
    Authors: Charras Et al.
    J Cell Biol
  27. Emergent patterns of growth controlled by multicellular form and mechanics.
    Authors: Nelson Et al.
    Am J Physiol Heart Circ Physiol
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