Chemical Name: (±)-1,2,3,3a-Tetrahydro-3a-hydroxy-6-methyl-1-phenyl-4H-pyrrolo[2,3-b]quinolin-4-one
Biological Activity(±)-Blebbistatin is a selective inhibitor of myosin II ATPase activity; inhibits contraction of the cleavage furrow without disrupting mitosis or contractile ring assembly. (�)-Blebbistatin rapidly and reversibly blocks cell blebbing, and disrupts directed cell migration and cytokinesis in vertebrate cells. (�)-Blebbistatin inhibits arterial thrombosis development, reduces infiltration of inflammatory cells, and reduces damage to vascular tissue in vivo in a mice model of arterial thrombosis.
Active and Inactive Enantiomer also available.
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Non-muscle myosin II regulates survival threshold of pluripotent stem cells.
Walker et al.
Specificity of blebbistatin, an inhibitor of myosin II.
Limouze et al.
J.Muscle Res.Cell Motil., 2004;25:337
Dissecting temporal and spatial control of cytokinesis with a myosin II inhibitor.
Straight et al.
Dissecting cytokinesis with small molecules.
Straight et al.
Amer.Soc.Cell Biol.Abstr., 2002;:2515
Citations for (±)-Blebbistatin
The citations listed below are publications that use Tocris products. Selected citations for (±)-Blebbistatin include:
44 Citations: Showing 1 - 10
Nuclear envelope deformation controls cell cycle progression in response to mechanical force.
Authors: Aureille Et al.
EMBO Rep 2019;20:e48084
Volume expansion and TRPV4 activation regulate stem cell fate in three-dimensional microenvironments.
Authors: Lee Et al.
Nat Commun 2019;10:529
Microtubule-Based Control of Motor-Clutch System Mechanics in Glioma Cell Migration.
Authors: Prahl Et al.
Cell Rep 2018;25:2591
ROCK signaling promotes collagen remodeling to facilitate invasive pancreatic ductal adenocarcinoma tumor cell growth.
Authors: Rath Et al.
EMBO Mol Med 2017;9:198
MYH9 is an Essential Factor for Porcine Reproductive and Respiratory Syndrome Virus Infection.
Authors: Gao Et al.
Nat Commun 2016;6:25120
Real-time two- and three-dimensional imaging of monocyte motility and navigation on planar surfaces and in collagen matrices: roles of Rho.
Authors: Bzymek Et al.
Sci Rep 2016;6:25016
Myocardial Infarction Causes Transient Cholinergic Transdifferentiation of Cardiac Sympathetic Nerves via gp130.
Authors: Olivas Et al.
J Neurosci 2016;36:479
Cell migration and antigen capture are antagonistic processes coupled by myosin II in dendritic cells.
Authors: Chabaud Et al.
Nat Commun 2015;6:7526
Substrate stress relaxation regulates cell spreading.
Authors: Chaudhuri Et al.
Int J Nanomedicine 2015;6:6364
Targeting protein tyrosine phosphatase σ after myocardial infarction restores cardiac sympathetic innervation and prevents arrhythmias.
Authors: Gardner Et al.
Nat Commun 2015;6:6235
Expression of α-Smooth Muscle Actin Determines the Fate of Mesenchymal Stromal Cells.
Authors: Talele Et al.
Stem Cell Reports 2015;4:1016
Mechanical coupling between transsynaptic N-cadherin adhesions and actin flow stabilizes dendritic spines.
Authors: Chazeau Et al.
Int J Cancer 2015;26:859
ZO-1 controls endothelial adherens junctions, cell-cell tension, angiogenesis, and barrier formation.
Authors: Tornavaca Et al.
Cortical contractility triggers a stochastic switch to fast amoeboid cell motility.
Authors: Ruprecht Et al.
PLoS One 2015;160:673
SDF-1α stiffens myeloma bone marrow mesenchymal stromal cells through the activation of RhoA-ROCK-Myosin II.
Authors: Choi Et al.
J Cell Biol 2015;136:E219
Atherosclerosis exacerbates arrhythmia following myocardial infarction: Role of myocardial inflammation.
Authors: Jesus Et al.
Mol Biol Cell 2015;12:169
Role of sarcoplasmic reticulum calcium in development of secondary calcium rise and early afterdepolarizations in long QT syndrome rabbit model.
Authors: Chang Et al.
J Cell Sci 2015;10:e0123868
SimV. enhances Rho/actin/cell rigidity pathway contributing to mesenchymal stem cells' osteogenic differentiation.
Authors: Tai Et al.
Heart Rhythm 2015;10:5881
Live Cell Interferometry Quantifies Dynamics of Biomass Partitioning during Cytokinesis.
Authors: Zangle Et al.
PLoS One 2014;9:e115726
Optical mapping of sarcoplasmic reticulum Ca2+ in the intact heart: ryanodine receptor refractoriness during alternans and fibrillation.
Authors: Wang Et al.
Circ Res 2014;114:1410
Organ explant culture of neonatal rat ventricles: a new model to study gene and cell therapy.
Authors: Haan Et al.
PLoS One 2013;8:e59290
Sinoatrial node reentry in a canine chronic left ventricular infarct model: role of intranodal fibrosis and heterogeneity of refractoriness.
Authors: Glukhov Et al.
Circ Arrhythm Electrophysiol 2013;6:984
Functional roles of KATP channel subunits in metabolic inhibition.
Authors: Glukhov Et al.
J Mol Cell Cardiol 2013;62:90
Mitochondrial dysfunction causing cardiac sodium channel downregulation in cardiomyopathy.
Authors: Liu Et al.
J Mol Cell Cardiol 2013;54:25
Diabetic hyperglycaemia activates CaMKII and arrhythmias by O-linked glycosylation.
Authors: Erickson Et al.
Three-dimensional printing physiology laboratory technology.
Authors: Sulkin Et al.
Am J Physiol Heart Circ Physiol 2013;305:H1569
Shaping organs by a wingless-int/Notch/nonmuscle myosin module which orients feather bud elongation.
Authors: Li Et al.
Proc Natl Acad Sci U S A 2013;110:E1452
EGFR and myosin II inhibitors cooperate to suppress EGFR-T790M-mutant NSCLC cells.
Authors: Chiu Et al.
Mol Oncol 2012;6:299
LTP induction translocates cortactin at distant synapses in wild-type but not Fmr1 knock-out mice.
Authors: Seese Et al.
J Neurosci 2012;32:7403
Intramural optical mapping of V(m) and Ca(i)2+ during long-duration ventricular fibrillation in canine hearts.
Authors: Kong Et al.
Am J Physiol Heart Circ Physiol 2012;302:H1294
CD36 protein influences myocardial Ca2+ homeostasis and phospholipid metabolism: conduction anomalies in CD36-deficient mice during fasting.
Authors: Pietka Et al.
J Biol Chem 2012;287:38901
A Rho GTPase signal treadmill backs a contractile array.
Authors: Burkel Et al.
Dev Cell 2012;23:384
Contractile Activity Regulates Inducible Nitric Oxide Synthase Expression and NO(i) Production in Cardiomyocytes via a FAK-Dependent Signaling Pathway.
Authors: Chu Et al.
J Signal Transduct 2012;2012:473410
Effects of KATP channel openers Dox and pinacidil in coronary-perfused atria and ventricles from failing and non-failing human hearts.
Authors: Fedorov Et al.
J Mol Cell Cardiol 2011;51:215
RhoJ/TCL regulates endothelial motility and tube formation and modulates actomyosin contractility and focal adhesion numbers.
Authors: Kaur Et al.
Arterioscler Thromb Vasc Biol 2011;31:657
Role of Pyk2 in cardiac arrhythmogenesis.
Authors: Lang Et al.
J Inflamm (Lond) 2011;301:H975
'White wave' analysis of epithelial scratch wound healing reveals how cells mobilise back from the leading edge in a myosin-II-dependent fashion.
Authors: Matsubayashi Et al.
Proc Natl Acad Sci U S A 2011;124:1017
Transmural heterogeneity and remodeling of ventricular excitation-contraction coupling in human heart failure.
Authors: Lou Et al.
Optical mapping of action potentials and calcium transients in the mouse heart.
Authors: Lang Et al.
J Vis Exp 2011;
Flotillins interact with PSGL-1 in neutrophils and, upon stimulation, rapidly organize into membrane domains subsequently accumulating in the uropod.
Authors: Rossy Et al.
PLoS One 2009;4:e5403
Emergence of patterned stem cell differentiation within multicellular structures.
Authors: Ruiz and Chen
Sci Rep 2008;26:2921
Rac1-null mouse embryonic fibroblasts are motile and respond to platelet-derived growth factor.
Authors: Vidali Et al.
Mol Biol Cell 2006;17:2377
Reassembly of contractile actin cortex in cell blebs.
Authors: Charras Et al.
J Cell Biol 2006;175:477
Emergent patterns of growth controlled by multicellular form and mechanics.
Authors: Nelson Et al.
Am J Physiol Heart Circ Physiol 2005;102:11594
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