Catalog Number: 1760
Chemical Name: (±)-1,2,3,3a-Tetrahydro-3a-hydroxy-6-methyl-1-phenyl-4H-pyrrolo[2,3-b]quinolin-4-one
Biological Activity
Selective inhibitor of myosin II ATPase activity; inhibits contraction of the cleavage furrow without disrupting mitosis or contractile ring assembly. Rapidly and reversibly blocks cell blebbing, and disrupts directed cell migration and cytokinesis in vertebrate cells.

Active and Inactive Enantiomer also available.

Technical Data
  • M.Wt:
  • Formula:
  • Solubility:
    Soluble to 100 mM in DMSO
  • Purity:
  • Storage:
    Store at +4°C
  • CAS No:
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis. All Tocris products are intended for laboratory research use only.
Additional Information
Other Product-Specific Information:
Background References
  1. Specificity of blebbistatin, an inhibitor of myosin II.
    Limouze et al.
    J.Muscle Res.Cell Motil., 2004;25:337
  2. Dissecting temporal and spatial control of cytokinesis with a myosin II inhibitor.
    Straight et al.
    Science, 2003;299:1743
  3. Non-muscle myosin II regulates survival threshold of pluripotent stem cells.
    Walker et al.
    Nat.Commun., 2010;1:71
  4. Dissecting cytokinesis with small molecules.
    Straight et al.
    Amer.Soc.Cell Biol.Abstr., 2002;:2515

The citations listed below are publications that use Tocris products. Selected citations for (±)-Blebbistatin include:

27 Citations: Showing 1 - 10
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  1. MYH9 is an Essential Factor for Porcine Reproductive and Respiratory Syndrome Virus Infection.
    Authors: Gao Et al.
    Nat Commun 2016;6:25120
  2. Substrate stress relaxation regulates cell spreading.
    Authors: Chaudhuri Et al.
    Int J Nanomedicine 2015;6:6364
  3. Expression of α-Smooth Muscle Actin Determines the Fate of Mesenchymal Stromal Cells.
    Authors: Talele Et al.
    Stem Cell Reports 2015;4:1016
  4. Mechanical coupling between transsynaptic N-cadherin adhesions and actin flow stabilizes dendritic spines.
    Authors: Chazeau Et al.
    Int J Cancer 2015;26:859
  5. ZO-1 controls endothelial adherens junctions, cell-cell tension, angiogenesis, and barrier formation.
    Authors: Tornavaca Et al.
    Cell 2015;208:821
  6. Cortical contractility triggers a stochastic switch to fast amoeboid cell motility.
    Authors: Ruprecht Et al.
    PLoS One 2015;160:673
  7. SDF-1α stiffens myeloma bone marrow mesenchymal stromal cells through the activation of RhoA-ROCK-Myosin II.
    Authors: Choi Et al.
    J Cell Biol 2015;136:E219
  8. Atherosclerosis exacerbates arrhythmia following myocardial infarction: Role of myocardial inflammation.
    Authors: Jesus Et al.
    Mol Biol Cell 2015;12:169
  9. Role of sarcoplasmic reticulum calcium in development of secondary calcium rise and early afterdepolarizations in long QT syndrome rabbit model.
    Authors: Chang Et al.
    J Cell Sci 2015;10:e0123868
  10. Simvastatin enhances Rho/actin/cell rigidity pathway contributing to mesenchymal stem cells' osteogenic differentiation.
    Authors: Tai Et al.
    Heart Rhythm 2015;10:5881
  11. Live Cell Interferometry Quantifies Dynamics of Biomass Partitioning during Cytokinesis.
    Authors: Zangle Et al.
    PLoS One 2014;9:e115726
  12. Organ explant culture of neonatal rat ventricles: a new model to study gene and cell therapy.
    Authors: Haan Et al.
    PLoS One 2013;8:e59290
  13. Sinoatrial node reentry in a canine chronic left ventricular infarct model: role of intranodal fibrosis and heterogeneity of refractoriness.
    Authors: Glukhov Et al.
    Circ Arrhythm Electrophysiol 2013;6:984
  14. Mitochondrial dysfunction causing cardiac sodium channel downregulation in cardiomyopathy.
    Authors: Liu Et al.
    J Mol Cell Cardiol 2013;54:25
  15. LTP induction translocates cortactin at distant synapses in wild-type but not Fmr1 knock-out mice.
    Authors: Seese Et al.
    J Neurosci 2012;32:7403
  16. A Rho GTPase signal treadmill backs a contractile array.
    Authors: Burkel Et al.
    Dev Cell 2012;23:384
  17. Contractile Activity Regulates Inducible Nitric Oxide Synthase Expression and NO(i) Production in Cardiomyocytes via a FAK-Dependent Signaling Pathway.
    Authors: Chu Et al.
    J Signal Transduct 2012;2012:473410
  18. Effects of KATP channel openers diazoxide and pinacidil in coronary-perfused atria and ventricles from failing and non-failing human hearts.
    Authors: Fedorov Et al.
    J Mol Cell Cardiol 2011;51:215
  19. RhoJ/TCL regulates endothelial motility and tube formation and modulates actomyosin contractility and focal adhesion numbers.
    Authors: Kaur Et al.
    Arterioscler Thromb Vasc Biol 2011;31:657
  20. Role of Pyk2 in cardiac arrhythmogenesis.
    Authors: Lang Et al.
    J Inflamm (Lond) 2011;301:H975
  21. 'White wave' analysis of epithelial scratch wound healing reveals how cells mobilise back from the leading edge in a myosin-II-dependent fashion.
    Authors: Matsubayashi Et al.
    Proc Natl Acad Sci U S A 2011;124:1017
  22. Transmural heterogeneity and remodeling of ventricular excitation-contraction coupling in human heart failure.
    Authors: Lou Et al.
    Circulation 2011;123:1881
  23. Optical mapping of action potentials and calcium transients in the mouse heart.
    Authors: Lang Et al.
    J Vis Exp 2011;
  24. Emergence of patterned stem cell differentiation within multicellular structures.
    Authors: Ruiz and Chen
    Sci Rep 2008;26:2921
  25. Rac1-null mouse embryonic fibroblasts are motile and respond to platelet-derived growth factor.
    Authors: Vidali Et al.
    Mol Biol Cell 2006;17:2377
  26. Reassembly of contractile actin cortex in cell blebs.
    Authors: Charras Et al.
    J Cell Biol 2006;175:477
  27. Emergent patterns of growth controlled by multicellular form and mechanics.
    Authors: Nelson Et al.
    Am J Physiol Heart Circ Physiol 2005;102:11594
Expand to show all 27 Citations
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