BLNK Antibody (5G9)

Novus Biologicals | Catalog # NBP2-37585

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5G9
View all BLNK Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human BLNK expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BLNK Antibody (5G9)

Western Blot: BLNK Antibody (5G9)BSA Free [NBP2-37585]

Western Blot: BLNK Antibody (5G9)BSA Free [NBP2-37585]

Western Blot: BLNK Antibody (5G9) [NBP2-37585] - Western blot analysis using BLNK mouse mAb against NIH/3T3 (1) and BCBL-1 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: BLNK Antibody (5G9) - BSA Free [NBP2-37585]

Immunocytochemistry/ Immunofluorescence: BLNK Antibody (5G9) - BSA Free [NBP2-37585]

Immunocytochemistry/Immunofluorescence: BLNK Antibody (5G9) [NBP2-37585] - Immunofluorescence analysis of HepG2 cells using BLNK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: BLNK Antibody (5G9) - BSA Free [NBP2-37585]

Immunohistochemistry-Paraffin: BLNK Antibody (5G9) - BSA Free [NBP2-37585]

Immunohistochemistry-Paraffin: BLNK Antibody (5G9) [NBP2-37585] - Analysis of human cervical cancer tissues using BLNK mouse mAb with DAB staining.
Flow Cytometry: BLNK Antibody (5G9) - BSA Free [NBP2-37585]

Flow Cytometry: BLNK Antibody (5G9) - BSA Free [NBP2-37585]

Flow Cytometry: BLNK Antibody (5G9) [NBP2-37585] - Flow cytometric analysis of NIH/3T3 cells using BLNK mouse mAb (green) and negative control (purple).
Western Blot: BLNK Antibody (5G9)BSA Free [NBP2-37585]

Western Blot: BLNK Antibody (5G9)BSA Free [NBP2-37585]

Western Blot: BLNK Antibody (5G9) [NBP2-37585] - Western blot analysis using BLNK mAb against human BLNK (AA: 34-216) recombinant protein. (Expected MW is 60 kDa)
ELISA: BLNK Antibody (5G9) - BSA Free [NBP2-37585]

ELISA: BLNK Antibody (5G9) - BSA Free [NBP2-37585]

ELISA: BLNK Antibody (5G9) [NBP2-37585] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for BLNK Antibody (5G9)

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BLNK

B-cell linker protein (BLNK, also known as SLP65, BASH, and BCA) is a B cell adaptor that plays an important role in B cell development and in BCR-signal transduction. Since BLNK does not encode any intrinsic enzymatic activity, its function is to serve as a scaffold for assembling macromolecular complexes which includes enzymes (PLC gamma, Vav and Btk) and additional linker proteins (Grb2 and Nck) (1-3). In addition, BLNK binds Btk and is required for activation of the transcription factor NF-

Long Name

B-cell Linker Protein

Alternate Names

BASH, Ly57, SLP65

Gene Symbol

BLNK

Additional BLNK Products

Product Documents for BLNK Antibody (5G9)

Certificate of Analysis

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Product Specific Notices for BLNK Antibody (5G9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for BLNK Antibody (5G9)

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Protocols

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FAQs

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