BLZF1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-83082

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all BLZF1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KSLGHHKGEFLGQSEGVIEPNKELSEVKNVLEKLKNSERRLLQDKEGLSNQLRVQTEVNRELKKLLVASVGDDLQYHFERLARE

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (87%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BLZF1 Antibody - BSA Free

Western Blot: BLZF1 Antibody [NBP1-83082]

Western Blot: BLZF1 Antibody [NBP1-83082]

Western Blot: BLZF1 Antibody [NBP1-83082] - Analysis in control (vector only transfected HEK293T lysate) and BLZF1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: BLZF1 Antibody [NBP1-83082]

Immunohistochemistry-Paraffin: BLZF1 Antibody [NBP1-83082]

Immunohistochemistry-Paraffin: BLZF1 Antibody [NBP1-83082] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine cells.
Knockdown Validated: BLZF1 Antibody [NBP1-83082]

Western Blot: BLZF1 Antibody [NBP1-83082]

Western Blot: BLZF1 Antibody [NBP1-83082] - Analysis in human cell line A-431 and human cell line MCF-7.

Applications for BLZF1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BLZF1

BLZF1 is required for normal Golgi structure and for protein transport from the endoplasmic reticulum (ER) through the Golgi apparatus to the cell surface

Alternate Names

basic leucine zipper nuclear factor 1JEM-1s, cytoplasmic protein, GOLGIN-45, JEM1, JEM-1MGC22497, JEM-1short protein, p45 basic leucine-zipper nuclear factor

Gene Symbol

BLZF1

Additional BLZF1 Products

Product Documents for BLZF1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for BLZF1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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