BMP-6 Antibody (6N5S9)

Novus Biologicals | Catalog # NBP3-16430

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6N5S9 expressed in HEK293
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Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 350-450 of human BMP-6 (NP_001709.1). DGPYDKQPFMVAFFKVSEVHVRTTRSASSRRRQQSRNRSTQSQDVARVSSASDYNSSELKTACRKHELYVSFQDLGWQDWIIAPKGYAANYCDGECSFPLN

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BMP-6 Antibody (6N5S9)

Western Blot: BMP-6 Antibody (6N5S9) [NBP3-16430]

Western Blot: BMP-6 Antibody (6N5S9) [NBP3-16430]

Western Blot: BMP-6 Antibody (6N5S9) [NBP3-16430] - Western blot analysis of extracts of various cell lines, using BMP-6 Rabbit mAb (NBP3-16430) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Immunocytochemistry/ Immunofluorescence: BMP-6 Antibody (6N5S9) [NBP3-16430]

Immunocytochemistry/ Immunofluorescence: BMP-6 Antibody (6N5S9) [NBP3-16430]

Immunocytochemistry/Immunofluorescence: BMP-6 Antibody (6N5S9) [NBP3-16430] - Immunofluorescence analysis of U-2 OS cells using BMP-6 Rabbit mAb (NBP3-16430) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for BMP-6 Antibody (6N5S9)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL.

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:1000 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BMP-6

The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based on its expression early in embryogenesis, the BMP encoded by this gene has a proposed role in early development. In addition, the fact that this BMP is closely related to BMP5 and BMP7 has lead to speculation of possible bone inductive activity.

Long Name

Bone Morphogenetic Protein 6

Alternate Names

BMP6, Vgr-1

Gene Symbol

BMP6

Additional BMP-6 Products

Product Documents for BMP-6 Antibody (6N5S9)

Certificate of Analysis

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Product Specific Notices for BMP-6 Antibody (6N5S9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for BMP-6 Antibody (6N5S9)

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Protocols

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