BOB1 Antibody (5J7A6)

Novus Biologicals | Catalog # NBP3-57398

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5J7A6
View all BOB1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide. This information is considered to be commercially sensitive. (Swissprot# Q16633)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is highly specific and optimized for use in Immunohistochemistry.

Normal Tissues Tested
Fallopian tube, Pancreas, Lung, Kidney, Placenta, Stomach, Liver, Thyroid, Appendix, Testis, Prostate, Tonsil, Cervix, esophagus, Breast, Colon, Brain

Cancer Tissues Tested
Lung adenocarcinoma, Lung squamous carcinoma, Hepatocellular Carcinoma, Hepatocholangio carcinoma, Thyroid Cancer, Prostate cancer, cervical squamous cell carcinoma, Breast Cancer, Colon Cancer, B-cell lymphoma, follicular lymphoma

Positive Controls
Tonsil, spleen, B-cell lymphoma, follicular lymphoma

Negative Controls
Epithelial cells. tonsil (epithelial cells), Fallopian tube, Pancreas, Kidney, Placenta, Stomach, Liver, Thyroid, Brain

Scientific Data Images for BOB1 Antibody (5J7A6)

BOB1 Antibody (5J7A6) Immunocytochemistry/ Immunofluorescence: BOB1 Antibody (5J7A6) [NBP3-57398]

Immunocytochemistry/ Immunofluorescence: BOB1 Antibody (5J7A6) [NBP3-57398]

Confocal imaging of Raji cells (postive) and HeLa cells (negative) using NBP3-57398 (dilution 1:300) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with alpha-Tubulin Mouse mAb (dilution 1:400) followed by incubation with conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
BOB1 Antibody (5J7A6) Immunocytochemistry/ Immunofluorescence: BOB1 Antibody (5J7A6) [NBP3-57398]

Immunocytochemistry/ Immunofluorescence: BOB1 Antibody (5J7A6) [NBP3-57398]

Confocal imaging of Daudi cells using NBP3-57398 (dilution 1:300) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
BOB1 Antibody (5J7A6) Immunocytochemistry/ Immunofluorescence: BOB1 Antibody (5J7A6) [NBP3-57398]

Immunocytochemistry/ Immunofluorescence: BOB1 Antibody (5J7A6) [NBP3-57398]

Confocal imaging of A20 cells using NBP3-57398 (dilution 1:300) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
BOB1 Antibody (5J7A6) Western Blot: BOB1 Antibody (5J7A6) [NBP3-57398]

Western Blot: BOB1 Antibody (5J7A6) [NBP3-57398]

Western blot analysis of various lysates using NBP3-57398 at 1:12000 dilution incubated overnight at 4C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Negative control (NC): HeLa Exposure time: 1s.
BOB1 Antibody (5J7A6) Immunohistochemistry-Paraffin: BOB1 Antibody (5J7A6) [NBP3-57398]

Immunohistochemistry-Paraffin: BOB1 Antibody (5J7A6) [NBP3-57398]

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using NBP3-57398 at a dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
BOB1 Antibody (5J7A6) Immunohistochemistry-Paraffin: BOB1 Antibody (5J7A6) [NBP3-57398]

Immunohistochemistry-Paraffin: BOB1 Antibody (5J7A6) [NBP3-57398]

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using NBP3-57398 at a dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
BOB1 Antibody (5J7A6) Immunohistochemistry-Paraffin: BOB1 Antibody (5J7A6) [NBP3-57398]

Immunohistochemistry-Paraffin: BOB1 Antibody (5J7A6) [NBP3-57398]

Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using NBP3-57398 at a dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
BOB1 Antibody (5J7A6) Immunohistochemistry-Paraffin: BOB1 Antibody (5J7A6) [NBP3-57398]

Immunohistochemistry-Paraffin: BOB1 Antibody (5J7A6) [NBP3-57398]

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using NBP3-57398 at a dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
BOB1 Antibody (5J7A6) Immunohistochemistry-Paraffin: BOB1 Antibody (5J7A6) [NBP3-57398]

Immunohistochemistry-Paraffin: BOB1 Antibody (5J7A6) [NBP3-57398]

Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using NBP3-57398 at a dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
BOB1 Antibody (5J7A6) Immunohistochemistry-Paraffin: BOB1 Antibody (5J7A6) [NBP3-57398]

Immunohistochemistry-Paraffin: BOB1 Antibody (5J7A6) [NBP3-57398]

Immunohistochemistry analysis of paraffin-embedded tissues conducted under the same conditions: NBP3-57398 at a dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Applications for BOB1 Antibody (5J7A6)

Application
Recommended Usage

ELISA

1 ug/mL

Immunocytochemistry/ Immunofluorescence

1:200 - 1:400

Immunohistochemistry-Paraffin

1:400 - 1:1600

Western Blot

1:12000 - 1:72000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, (pH 7.3)

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BOB1

BOB1 is a novel 35kDa protein designated Bob1 (B cell Oct binding protein 1) is a lymphocyte specific transcription co activator protein. B cell specific transcription is mediated by octamer motifs found in immunoglobulin heavy and light chain gene promoters and in some immunoglobulin enhancers. These sites are bound by the B cell specific Oct1 and Oct2 transcription factors, and the B cell restricted co activator BOB.1/OBF.1. This protein interacts only with the Oct1/2 proteins through sub domains in the POU domain of the Oct1/2 proteins, enhancing their transcriptional efficacy. Although having no intrinsic capacity for DNA binding, Bob1 associates tightly with the octomer motif in the presence of Oct1 and Oct2. Bob1 is expressed at highest levels in spleen and peripheral blood leukocytes.

Alternate Names

B-cell-specific coactivator OBF-1, BOB-1, OBF-1, OBF1BOB1, OCAB, OCA-B, OCT-binding factor 1, POU class 2 associating factor 1, POU domain class 2, associating factor 1, POU domain class 2-associating factor 1, POU domain, class 2, associating factor 1

Gene Symbol

POU2AF1

Additional BOB1 Products

Product Documents for BOB1 Antibody (5J7A6)

Certificate of Analysis

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Product Specific Notices for BOB1 Antibody (5J7A6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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