Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI8A1
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Product Specifications
Immunogen
Full length human recombinant protein of human DLX4(NP_612138) produced in E.coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
26.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for BP1 Antibody (OTI8A1)
![Western Blot: BP1 Antibody (OTI8A1) [NBP2-46463]](https://resources.rndsystems.com/images/products/BP1-Antibody-8A1-Western-Blot-NBP2-46463-img0011.jpg "Western Blot: BP1 Antibody (OTI8A1) [NBP2-46463]")
Western Blot: BP1 Antibody (OTI8A1) [NBP2-46463]
Western Blot: BP1 Antibody (8A1) [NBP2-46463] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BP1.![Immunohistochemistry: BP1 Antibody (OTI8A1) [NBP2-46463]](https://resources.rndsystems.com/images/products/BP1-Antibody-8A1-Immunohistochemistry-NBP2-46463-img0010.jpg "Immunohistochemistry: BP1 Antibody (OTI8A1) [NBP2-46463]")
Immunohistochemistry: BP1 Antibody (OTI8A1) [NBP2-46463]
Immunohistochemistry: BP1 Antibody (8A1) [NBP2-46463] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)![Immunohistochemistry: BP1 Antibody (OTI8A1) [NBP2-46463]](https://resources.rndsystems.com/images/products/BP1-Antibody-8A1-Immunohistochemistry-NBP2-46463-img0001.jpg "Immunohistochemistry: BP1 Antibody (OTI8A1) [NBP2-46463]")
Immunohistochemistry: BP1 Antibody (OTI8A1) [NBP2-46463]
Immunohistochemistry: BP1 Antibody (8A1) [NBP2-46463] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)![Immunohistochemistry: BP1 Antibody (OTI8A1) [NBP2-46463]](https://resources.rndsystems.com/images/products/BP1-Antibody-8A1-Immunohistochemistry-NBP2-46463-img0002.jpg "Immunohistochemistry: BP1 Antibody (OTI8A1) [NBP2-46463]")
Immunohistochemistry: BP1 Antibody (OTI8A1) [NBP2-46463]
Immunohistochemistry: BP1 Antibody (8A1) [NBP2-46463] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)![Immunohistochemistry: BP1 Antibody (OTI8A1) [NBP2-46463]](https://resources.rndsystems.com/images/products/BP1-Antibody-8A1-Immunohistochemistry-NBP2-46463-img0003.jpg "Immunohistochemistry: BP1 Antibody (OTI8A1) [NBP2-46463]")
Immunohistochemistry: BP1 Antibody (OTI8A1) [NBP2-46463]
Immunohistochemistry: BP1 Antibody (8A1) [NBP2-46463] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)![Immunohistochemistry: BP1 Antibody (OTI8A1) [NBP2-46463]](https://resources.rndsystems.com/images/products/BP1-Antibody-8A1-Immunohistochemistry-NBP2-46463-img0004.jpg "Immunohistochemistry: BP1 Antibody (OTI8A1) [NBP2-46463]")
Immunohistochemistry: BP1 Antibody (OTI8A1) [NBP2-46463]
Immunohistochemistry: BP1 Antibody (8A1) [NBP2-46463] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)![Immunohistochemistry: BP1 Antibody (OTI8A1) [NBP2-46463]](https://resources.rndsystems.com/images/products/BP1-Antibody-8A1-Immunohistochemistry-NBP2-46463-img0005.jpg "Immunohistochemistry: BP1 Antibody (OTI8A1) [NBP2-46463]")
Immunohistochemistry: BP1 Antibody (OTI8A1) [NBP2-46463]
Immunohistochemistry: BP1 Antibody (8A1) [NBP2-46463] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)![Immunohistochemistry: BP1 Antibody (OTI8A1) [NBP2-46463]](https://resources.rndsystems.com/images/products/BP1-Antibody-8A1-Immunohistochemistry-NBP2-46463-img0006.jpg "Immunohistochemistry: BP1 Antibody (OTI8A1) [NBP2-46463]")
Immunohistochemistry: BP1 Antibody (OTI8A1) [NBP2-46463]
Immunohistochemistry: BP1 Antibody (8A1) [NBP2-46463] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)![Immunohistochemistry: BP1 Antibody (OTI8A1) [NBP2-46463]](https://resources.rndsystems.com/images/products/BP1-Antibody-8A1-Immunohistochemistry-NBP2-46463-img0007.jpg "Immunohistochemistry: BP1 Antibody (OTI8A1) [NBP2-46463]")
Immunohistochemistry: BP1 Antibody (OTI8A1) [NBP2-46463]
Immunohistochemistry: BP1 Antibody (8A1) [NBP2-46463] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)![Immunohistochemistry: BP1 Antibody (OTI8A1) [NBP2-46463]](https://resources.rndsystems.com/images/products/BP1-Antibody-8A1-Immunohistochemistry-NBP2-46463-img0008.jpg "Immunohistochemistry: BP1 Antibody (OTI8A1) [NBP2-46463]")
Immunohistochemistry: BP1 Antibody (OTI8A1) [NBP2-46463]
Immunohistochemistry: BP1 Antibody (8A1) [NBP2-46463] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)![Immunohistochemistry: BP1 Antibody (OTI8A1) [NBP2-46463]](https://resources.rndsystems.com/images/products/BP1-Antibody-8A1-Immunohistochemistry-NBP2-46463-img0009.jpg "Immunohistochemistry: BP1 Antibody (OTI8A1) [NBP2-46463]")
Immunohistochemistry: BP1 Antibody (OTI8A1) [NBP2-46463]
Immunohistochemistry: BP1 Antibody (8A1) [NBP2-46463] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)Applications for BP1 Antibody (OTI8A1)
Application
Recommended Usage
Immunohistochemistry
1:150
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: BP1
BP1 has been shown to be activated in 80% of invasive ductal breast tumors and is associated with breast cancer progression. BP1 is also expressed in the bone marrows of 63% of acute myeloid leukemia (AML) patients. In addition, BP1 plays a role in globin gene regulation by repressing the beta-globin gene. Therefore, BP1 antibodies are useful tools for various cancer studies.
Alternate Names
Beta protein 1, BP1distal-less homeo box 7, distal-less homeobox 4, DLX7distal-less homeo box 9, DLX8DLX9, homeobox protein DLX-4, Homeobox protein DLX-7, Homeobox protein DLX-8
Gene Symbol
DLX4
Additional BP1 Products
Product Documents for BP1 Antibody (OTI8A1)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for BP1 Antibody (OTI8A1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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