BP1 Antibody (OTI8A1) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-70581

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI8A1

Format

Azide and BSA Free
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Product Specifications

Immunogen

Full length human recombinant protein of human DLX4(NP_612138) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

26.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BP1 Antibody (OTI8A1) - Azide and BSA Free

Western Blot: BP1 Antibody (OTI8A1)Azide and BSA Free [NBP2-70581]

Western Blot: BP1 Antibody (OTI8A1)Azide and BSA Free [NBP2-70581]

Western Blot: BP1 Antibody (OTI8A1) - Azide and BSA Free [NBP2-70581] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BP1.
Immunohistochemistry: BP1 Antibody (OTI8A1) - Azide and BSA Free [NBP2-70581]

Immunohistochemistry: BP1 Antibody (OTI8A1) - Azide and BSA Free [NBP2-70581]

Immunohistochemistry: BP1 Antibody (OTI8A1) - Azide and BSA Free [NBP2-70581] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: BP1 Antibody (OTI8A1) - Azide and BSA Free [NBP2-70581]

Immunohistochemistry: BP1 Antibody (OTI8A1) - Azide and BSA Free [NBP2-70581]

Immunohistochemistry: BP1 Antibody (OTI8A1) - Azide and BSA Free [NBP2-70581] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: BP1 Antibody (OTI8A1) - Azide and BSA Free [NBP2-70581]

Immunohistochemistry: BP1 Antibody (OTI8A1) - Azide and BSA Free [NBP2-70581]

Immunohistochemistry: BP1 Antibody (OTI8A1) - Azide and BSA Free [NBP2-70581] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: BP1 Antibody (OTI8A1) - Azide and BSA Free [NBP2-70581]

Immunohistochemistry: BP1 Antibody (OTI8A1) - Azide and BSA Free [NBP2-70581]

Immunohistochemistry: BP1 Antibody (OTI8A1) - Azide and BSA Free [NBP2-70581] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: BP1 Antibody (OTI8A1) - Azide and BSA Free [NBP2-70581]

Immunohistochemistry: BP1 Antibody (OTI8A1) - Azide and BSA Free [NBP2-70581]

Immunohistochemistry: BP1 Antibody (OTI8A1) - Azide and BSA Free [NBP2-70581] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: BP1 Antibody (OTI8A1) - Azide and BSA Free [NBP2-70581]

Immunohistochemistry: BP1 Antibody (OTI8A1) - Azide and BSA Free [NBP2-70581]

Immunohistochemistry: BP1 Antibody (OTI8A1) - Azide and BSA Free [NBP2-70581] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: BP1 Antibody (OTI8A1) - Azide and BSA Free [NBP2-70581]

Immunohistochemistry: BP1 Antibody (OTI8A1) - Azide and BSA Free [NBP2-70581]

Immunohistochemistry: BP1 Antibody (OTI8A1) - Azide and BSA Free [NBP2-70581] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: BP1 Antibody (OTI8A1) - Azide and BSA Free [NBP2-70581]

Immunohistochemistry: BP1 Antibody (OTI8A1) - Azide and BSA Free [NBP2-70581]

Immunohistochemistry: BP1 Antibody (OTI8A1) - Azide and BSA Free [NBP2-70581] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: BP1 Antibody (OTI8A1) - Azide and BSA Free [NBP2-70581]

Immunohistochemistry: BP1 Antibody (OTI8A1) - Azide and BSA Free [NBP2-70581]

Immunohistochemistry: BP1 Antibody (OTI8A1) - Azide and BSA Free [NBP2-70581] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: BP1 Antibody (OTI8A1) - Azide and BSA Free [NBP2-70581]

Immunohistochemistry: BP1 Antibody (OTI8A1) - Azide and BSA Free [NBP2-70581]

Immunohistochemistry: BP1 Antibody (OTI8A1) - Azide and BSA Free [NBP2-70581] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Applications for BP1 Antibody (OTI8A1) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BP1

BP1 (beta protein 1) is a homeodomain-containing isoform of DLX4. Strong BP1 expression is seen in placenta and kidney tissue, with no expression found in any other tissue.

BP1 has been shown to be activated in 80% of invasive ductal breast tumors and is associated with breast cancer progression. BP1 is also expressed in the bone marrows of 63% of acute myeloid leukemia (AML) patients. In addition, BP1 plays a role in globin gene regulation by repressing the beta-globin gene. Therefore, BP1 antibodies are useful tools for various cancer studies.

Alternate Names

Beta protein 1, BP1distal-less homeo box 7, distal-less homeobox 4, DLX7distal-less homeo box 9, DLX8DLX9, homeobox protein DLX-4, Homeobox protein DLX-7, Homeobox protein DLX-8

Gene Symbol

DLX4

Additional BP1 Products

Product Documents for BP1 Antibody (OTI8A1) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for BP1 Antibody (OTI8A1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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