BPDE Assay Kit, 96 well
BPDE Assay Kit, 96 well Summary
BPDE is a frequently used biomarker of oxidative DNA damage and oxidative stress. To address this need, we offer a validated HT BPDE ELISA kit, a fast and sensitive immunoassay for the detection and quantitation of BPDE adduct in plasma, serum, and cell culture supernatant samples. This assay employs a pre-coated BPDE-HSA monoclonal antibody, a labeled Biotinylated BPDE-HSA, a Strep-HRP conjugate, and chemiluminescent detection substrates to construct a high-throughput assay flexible for your experimental design. Important features of the assay include: 1) chemiluminescent, non-radioactive format; 2) a high-throughput 96-strip well plate; 3) a dynamic range from 6.25-400 nM BPDE-HSA; and 4) a sensitivity of 5 nM BPDE-HSA.
Kit Contents
- BDPE-HSA Standard
- Assay Diluent
- Biotinylated BPDE-HSA
- Pre-coated 96-strip well plate
- Strep-HRP
- PeroxyGlow™ A
- PeroxyGlow™ B
Cultrex is a registered trademark of Trevigen, Inc. PeroxyGlow is trademark of Trevigen, Inc.
Specifications
Product Datasheets
Background: BPDE
Benzo[a]pyrene is a polycyclic aromatic hydrocarbon found in cigarette smoke, heavily polluted air, and smoked foods. A diol epoxide derivative of benzo[a]pyrene, 7b, 8a-dihydroxy-9a,10a-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (BPDE-I) has been implicated as a key benzo[a]pyrene metabolite involved in covalent modification of DNA.
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