Brachyury Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-24676

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Primate

Cited:

Human, Mouse

Predicted:

Canine (93%), Monkey (93%), Rat (93%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Brachyury Primary Antibodies »

Product Specifications

Immunogen

A portion of amino acids 350-400 of human Brachyury was used as the immunogen.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28132888).

Marker

Mesoderm Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Brachyury Antibody - BSA Free

Western Blot: Brachyury Antibody [NBP2-24676]

Western Blot: Brachyury Antibody [NBP2-24676]

Western Blot: Brachyury Antibody [NBP2-24676] - Analysis of Brachyury in U87 cell lysate. Lane 1 shows pre-immune sera, lane 2 shows this antibody tested at 2 ug/mL.
Immunohistochemistry-Paraffin: Brachyury Antibody [NBP2-24676]

Immunohistochemistry-Paraffin: Brachyury Antibody [NBP2-24676]

Immunohistochemistry-Paraffin: Brachyury Antibody [NBP2-24676] - Analysis of Brachyury in FFPE human brain tissue using an isotype control (top left) and this antibody (bottom left, right) at 5 ug/mL.

Applications for Brachyury Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20 - 1:1000

Immunohistochemistry-Paraffin

5 ug/mL

Western Blot

1 - 3 ug/mL

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Brachyury

Brachyury / Bry is encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells.

Alternate Names

brachyury protein, MGC104817, Protein T, SAVA, T brachyury (mouse) homolog, T brachyury homolog, T Brachyury Transcription Factor, T, brachyury homolog (mouse), T-Box Transcription Factor T, TBXT, TF, TFT

Gene Symbol

TBXT

UniProt

O15178

Additional Brachyury Products

Product Documents for Brachyury Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Brachyury Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Brachyury Antibody - BSA Free

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Protocols

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FAQs

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