BRD4 Antibody (BL-149-2H5)
Novus Biologicals | Catalog # NBP2-76393
Recombinant Monoclonal Antibody
![Western Blot: BRD4 Antibody (BL-149-2H5) [NBP2-76393]](https://resources.rndsystems.com/images/products/BRD4-Antibody-BL-149-2H5-Western-Blot-NBP2-76393-img0008.jpg "Western Blot: BRD4 Antibody (BL-149-2H5) [NBP2-76393]")
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Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation Sequencing
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # BL-149-2H5
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Product Specifications
Immunogen
Immunogen sequence between amino acids 1312 and 1362
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for BRD4 Antibody (BL-149-2H5)
Western Blot: BRD4 Antibody (BL-149-2H5) [NBP2-76393]
Western Blot: BRD4 Antibody (BL-149-2H5) [NBP2-76393] - Detection of mouse BRD4 by WB of BW5147.3 lysate. Antibody: BRD4 Antibody (BL-149-2H5). Secondary: HRP-conjugated goat anti-rabbit IgG.![Immunocytochemistry/ Immunofluorescence: BRD4 Antibody (BL-149-2H5) [NBP2-76393]](https://resources.rndsystems.com/images/products/BRD4-Antibody-BL-149-2H5-Immunocytochemistry-Immunofluorescence-NBP2-76393-img0002.jpg "Immunocytochemistry/ Immunofluorescence: BRD4 Antibody (BL-149-2H5) [NBP2-76393]")
Immunocytochemistry/ Immunofluorescence: BRD4 Antibody (BL-149-2H5) [NBP2-76393]
Immunocytochemistry/Immunofluorescence: BRD4 Antibody (BL-149-2H5) [NBP2-76393] - Antibody: Rabbit anti-BRD4 recombinant monoclonal [BL-149-2H5] (NBP2-76393). Secondary: DyLight 550-conjugated goat anti-rabbit IgG. Counterstain: Phalloidin Alexa Fluor 488-conjugated (green).![Immunohistochemistry-Paraffin: BRD4 Antibody (BL-149-2H5) [NBP2-76393]](https://resources.rndsystems.com/images/products/BRD4-Antibody-BL-149-2H5-Immunohistochemistry-Paraffin-NBP2-76393-img0011.jpg "Immunohistochemistry-Paraffin: BRD4 Antibody (BL-149-2H5) [NBP2-76393]")
Immunohistochemistry-Paraffin: BRD4 Antibody (BL-149-2H5) [NBP2-76393]
Immunohistochemistry-Paraffin: BRD4 Antibody (BL-149-2H5) [NBP2-76393] - Section of breast carcinoma. Antibody: Rabbit anti-BRD4 recombinant monoclonal antibody [BL-149-2H5] used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.![Flow Cytometry: BRD4 Antibody (BL-149-2H5) [NBP2-76393]](https://resources.rndsystems.com/images/products/BRD4-Antibody-BL-149-2H5-Flow-Cytometry-NBP2-76393-img0010.jpg "Flow Cytometry: BRD4 Antibody (BL-149-2H5) [NBP2-76393]")
Flow Cytometry: BRD4 Antibody (BL-149-2H5) [NBP2-76393]
Flow Cytometry: BRD4 Antibody (BL-149-2H5) [NBP2-76393] - Detection of human BRD4 (shaded) in KG-1 cells by flow cytometry. Antibody: BRD4 Antibody (BL-149-2H5) or isotype control (unshaded). Secondary: DyLight(R) 650-conjugated goat anti-rabbit IgG.
Chromatin Immunoprecipitation Sequencing: BRD4 Antibody (BL-149-2H5) [NBP2-76393] - Localization of human BRD4 binding sites in immunoprecipitates from Mia PaCa-2 cells by ChIP-Seq. Antibody: Rabbit anti-BRD4 recombinant monoclonal [BL-149-2H5] NBP2-76393.
![Western Blot: BRD4 Antibody (BL-149-2H5) [NBP2-76393]](https://resources.rndsystems.com/images/products/BRD4-Antibody-BL-149-2H5-Western-Blot-NBP2-76393-img0007.jpg "Western Blot: BRD4 Antibody (BL-149-2H5) [NBP2-76393]")
Western Blot: BRD4 Antibody (BL-149-2H5) [NBP2-76393]
Western Blot: BRD4 Antibody (BL-149-2H5) [NBP2-76393] - Detection of human BRD4 by WB of HeLa, HEK293T, Jurkat, MDA-MB-231, A-549, SW620, and SK-MEL-28 lysate. Antibody: BRD4 Antibody (BL-149-2H5). Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-eEF2.![Immunohistochemistry-Paraffin: BRD4 Antibody (BL-149-2H5) [NBP2-76393]](https://resources.rndsystems.com/images/products/BRD4-Antibody-BL-149-2H5-Immunohistochemistry-Paraffin-NBP2-76393-img0009.jpg "Immunohistochemistry-Paraffin: BRD4 Antibody (BL-149-2H5) [NBP2-76393]")
Immunohistochemistry-Paraffin: BRD4 Antibody (BL-149-2H5) [NBP2-76393]
Immunohistochemistry-Paraffin: BRD4 Antibody (BL-149-2H5) [NBP2-76393] - Detection of human BRD4 in FFPE Hela cells by ICC. Antibody: BRD4 Antibody (BL-149-2H5). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.![Immunoprecipitation: BRD4 Antibody (BL-149-2H5) [NBP2-76393]](https://resources.rndsystems.com/images/products/BRD4-Antibody-BL-149-2H5-Immunoprecipitation-NBP2-76393-img0006.jpg "Immunoprecipitation: BRD4 Antibody (BL-149-2H5) [NBP2-76393]")
Immunoprecipitation: BRD4 Antibody (BL-149-2H5) [NBP2-76393]
Immunoprecipitation: BRD4 Antibody (BL-149-2H5) [NBP2-76393] - Detection of human BRD4 by WB of immunoprecipitates from HeLa lysate. Antibodies: Rabbit anti-BRD4 recombinant monoclonal [BL-149-2H5] (NBP2-76393).Applications for BRD4 Antibody (BL-149-2H5)
Application
Recommended Usage
Flow Cytometry
Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl
Immunocytochemistry/ Immunofluorescence
1:25 - 1:250
Immunohistochemistry
1:100-1:500
Immunohistochemistry-Paraffin
1:100-1:500
Immunoprecipitation
20 ul/mg lysate
Western Blot
1:1000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 2 - 8 C / 1 year from date of receipt
Background: BRD4
Long Name
Bromodomain Containing 4
Alternate Names
HUNK1, HUNKI, MCAP
Gene Symbol
BRD4
Additional BRD4 Products
Product Documents for BRD4 Antibody (BL-149-2H5)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for BRD4 Antibody (BL-149-2H5)
This Product sold under License from Bethyl Laboratories, Inc.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for BRD4 Antibody (BL-149-2H5)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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