BRD4 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-18874
![Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-18874]](https://resources.rndsystems.com/images/products/BRD4-Antibody-Immunohistochemistry-Paraffin-NBP1-18874-img0034.jpg "Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-18874]")
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 1312 and 1362 of human bromodomain-containing protein 4 using the numbering given in entry NP_490597.1 (GeneID 23476).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for BRD4 Antibody - BSA Free
Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-18874]
Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-18874] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-BRD4 used at a dilution of 1:5,000 (0.2 ug/ml). Detection: DAB![Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-18874]](https://resources.rndsystems.com/images/products/BRD4-Antibody-Immunohistochemistry-Paraffin-NBP1-18874-img0033.jpg "Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-18874]")
Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-18874]
Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-18874] - FFPE section of mouse CT26 colon carcinoma. Antibody: Affinity purified rabbit anti-BRD4 used at a dilution of 1:1,000 (1 ug/ml). Detection: DAB![Immunoprecipitation: BRD4 Antibody [NBP1-18874]](https://resources.rndsystems.com/images/products/BRD4-Antibody-Immunoprecipitation-NBP1-18874-img0035.jpg "Immunoprecipitation: BRD4 Antibody [NBP1-18874]")
Immunoprecipitation: BRD4 Antibody [NBP1-18874]
Immunoprecipitation: BRD4 Antibody [NBP1-18874] - Detection of human BRD4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BRD4 antibody NBP1-18874 used for IP at 3 ug per reaction. BRD4 was also immunoprecipitated by a different rabbit anti-BRD4 antibody and rabbit anti-BRD4 recombinant monoclonal antibody [BL-149-2H5] (NBP2-76393). For blotting immunoprecipitated BRD4, NBP2-76393 was used at 1:1000. Detection: Chemiluminescence with an exposure time time of 1 seconds.Applications for BRD4 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:1000-1:5000
Immunohistochemistry-Paraffin
1:1000-1:5000
Immunoprecipitation
2-10 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: BRD4
Long Name
Bromodomain Containing 4
Alternate Names
HUNK1, HUNKI, MCAP
Entrez Gene IDs
23476 (Human)
Gene Symbol
BRD4
UniProt
Additional BRD4 Products
Product Documents for BRD4 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for BRD4 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for BRD4 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for BRD4 Antibody - BSA Free
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Q: Why is BRD4 detected at around 200kd when it is a 152kd protein? Additionally, I noticed another company's antibody detects a protein of around 85kd.
A: Our antibody is detecting the full length BRD4 protein 1362 amino acids in length, which corresponds to approximately 200kDa. This is also referred to as the long isoform of BRD4. The 85kDa band that you mention being detected by another antibody would most likely correspond to the short isoform of BRD4 which is the alternative splice variant. The size of the short isoform is 722 amino acids which corresponds to approximately 83.45kDa.
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