BRE Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32304

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Knockout Validated, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all BRE Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human BRE. The exact sequence is proprietary.

Localization

Cytoplasm, Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BRE Antibody - BSA Free

Knockout Validated: BRE Antibody [NBP1-32304]

Western Blot: BRE Antibody [NBP1-32304]

Western Blot: BRE Antibody [NBP1-32304] - Wild-type (WT) and BRCC45 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BRCC45 antibody. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Immunocytochemistry/ Immunofluorescence: BRE Antibody [NBP1-32304]

Immunocytochemistry/ Immunofluorescence: BRE Antibody [NBP1-32304]

Immunocytochemistry/Immunofluorescence: BRE Antibody [NBP1-32304] - analysis of HeLa, using BRE antibody at 1:200 dilution.
Western Blot: BRE Antibody [NBP1-32304]

Western Blot: BRE Antibody [NBP1-32304]

Western Blot: BRE Antibody [NBP1-32304] - Sample: 1000 ug 293T whole cell lysate/extract A. Control with 2. 5 ug of preimmune rabbit IgG, B. Immunoprecipitation of BRE protein by 2. 5 ug of BRE antibody 10% SDS-PAGE gel.
Western Blot: BRE Antibody [NBP1-32304]

Western Blot: BRE Antibody [NBP1-32304]

Western Blot: BRE Antibody [NBP1-32304] - 30 ug C8D30 whole cell lysate/extract 10% SDS-PAGE gel, antibody dilution 1:1000.
Western Blot: BRE Antibody [NBP1-32304]

Western Blot: BRE Antibody [NBP1-32304]

Western Blot: BRE Antibody [NBP1-32304] - A. 30 ug 293T whole cell lysate/extract, B. 30 ug A431 whole cell lysate/extract 10 % SDS-PAGE gel, antibody dilution 1:1000.
BRE Antibody - BSA Free

Western Blot: BRE Antibody [NBP1-32304] -

Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BRE antibody (NBP1-32304) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for BRE Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: BRE

BRE is a component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRC

Alternate Names

brain and reproductive organ-expressed (TNFRSF1A modulator), BRCA1/BRCA2-containing complex subunit 45, subunit 4

Gene Symbol

BABAM2

Additional BRE Products

Product Documents for BRE Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for BRE Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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