Breast carcinoma amplified sequence 3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-92931

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Breast carcinoma amplified sequence 3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 674-913 of human BCAS3 (NP_060149.3). LAGLVPPGSPGPITRHGSYDSLASDHSGQEDEEWLSQVEIVTHTGPHRRLWMGPQFQFKTIHPSGQTTVISSSSSVLQSHGPSDTPQPLLDFDTDDLDLNSLRIQPVRSDPVSMPGSSRPVSDRRGVSTVIDAASGTFDRSVTLLEVCGSWPEGFGLRHMSSMEHTEEGLRERLADAMAESPSRDVVGSGTELQREGSIETLSNSSGSTSGSIPRNFDGYRSPLPTNESQPLSLFPTGFP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Breast carcinoma amplified sequence 3 Antibody - BSA Free

Western Blot: Breast carcinoma amplified sequence 3 AntibodyBSA Free [NBP2-92931]

Western Blot: Breast carcinoma amplified sequence 3 AntibodyBSA Free [NBP2-92931]

Western Blot: Breast carcinoma amplified sequence 3 Antibody [NBP2-92931] - Analysis of extracts of various cell lines, using Breast carcinoma amplified sequence 3 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.
Immunocytochemistry/ Immunofluorescence: Breast carcinoma amplified sequence 3 Antibody - BSA Free [NBP2-92931]

Immunocytochemistry/ Immunofluorescence: Breast carcinoma amplified sequence 3 Antibody - BSA Free [NBP2-92931]

Immunocytochemistry/Immunofluorescence: Breast carcinoma amplified sequence 3 Antibody [NBP2-92931] - Immunofluorescence analysis of A-549 cells using Breast carcinoma amplified sequence 3 antibody (NBP2-92931). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: Breast carcinoma amplified sequence 3 Antibody - BSA Free [NBP2-92931]

Immunohistochemistry-Paraffin: Breast carcinoma amplified sequence 3 Antibody - BSA Free [NBP2-92931]

Immunohistochemistry-Paraffin: Breast carcinoma amplified sequence 3 Antibody [NBP2-92931] - Paraffin-embedded mouse kidney using Breast carcinoma amplified sequence 3.
Western Blot: Breast carcinoma amplified sequence 3 AntibodyBSA Free [NBP2-92931]

Western Blot: Breast carcinoma amplified sequence 3 AntibodyBSA Free [NBP2-92931]

Western Blot: Breast carcinoma amplified sequence 3 Antibody [NBP2-92931] - Analysis of 200ug extracts of HeLa cells, using Breast carcinoma amplified sequence 3 at a dilition of 1:1000.
Immunohistochemistry-Paraffin: Breast carcinoma amplified sequence 3 Antibody - BSA Free [NBP2-92931]

Immunohistochemistry-Paraffin: Breast carcinoma amplified sequence 3 Antibody - BSA Free [NBP2-92931]

Immunohistochemistry-Paraffin: Breast carcinoma amplified sequence 3 Antibody [NBP2-92931] - Paraffin-embedded human kidney cancer using Breast carcinoma amplified sequence 3.
Immunohistochemistry-Paraffin: Breast carcinoma amplified sequence 3 Antibody - BSA Free [NBP2-92931]

Immunohistochemistry-Paraffin: Breast carcinoma amplified sequence 3 Antibody - BSA Free [NBP2-92931]

Immunohistochemistry-Paraffin: Breast carcinoma amplified sequence 3 Antibody [NBP2-92931] - Paraffin-embedded human stomach using Breast carcinoma amplified sequence 3.

Applications for Breast carcinoma amplified sequence 3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:50-1:100

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Breast carcinoma amplified sequence 3

BCAS3 acts as an ERalpha coactivator in breast cancer cells. It has been demonstrated that PELP1, a newly described ERalpha coregulator, is recruited to BCAS3 chromatin and activates its expression. Analysis of the BCAS3 sequence for functional motifs and evidence from biochemical fractionation suggested that BCAS3 acts as a transcriptional coactivator. Results from chromatin immunoprecipitation, reporter assays, and expression studies further validated the coactivator function of BCAS3 for ERalpha. BCAS3 physically associated with histone H3 and histone acetyltransferase complex protein P/CAF and possessed histone acetyltransferase activity.

Alternate Names

BCAS4/BCAS3 fusion, breast carcinoma amplified sequence 3, breast carcinoma amplified sequence 4/3 fusion protein, breast carcinoma-amplified sequence 3, DKFZp686O1527, FLJ20128, GAOB1, MAAB, metastasis associated antigen of breast cancer, MGC4973, protein Maab1

Gene Symbol

BCAS3

Additional Breast carcinoma amplified sequence 3 Products

Product Documents for Breast carcinoma amplified sequence 3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Breast carcinoma amplified sequence 3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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