Catalog Number: 1231
Chemical Name: 1,6,7,8,9,11aβ,12,13,14,14αa-Decahydro-1β,13α-dihydroxy-6β-methyl-4H-cyclopent(f)oxacyclotridecin-4-one
Biological Activity
Reversible inhibitor of protein translocation from the endoplasmic reticulum (ER) to the Golgi apparatus. Blocks binding of ADP-ribosylation factor to the Golgi apparatus and inhibits GDP-GTP exchange. Can be used to induce autophagy in mammalian cells. Also enhances CRISPR-mediated homology-directed repair (HDR) efficiency ~2-fold when applied at 100 nM, in human induced pluripotent stem cells (iPSCs).
Technical Data
  • M.Wt:
  • Formula:
  • Solubility:
    Soluble to 50 mM in DMSO and to 10 mM in ethanol
  • Purity:
  • Storage:
    Desiccate at -20°C
  • CAS No:
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis. All Tocris products are intended for laboratory research use only.
Background References
  1. Small Molecules Enhance CRISPR Genome Editing in Pluripotent Stem Cells.
    Yu et al.
    Cell Stem Cell, 2015;16:142
  2. Action of brefeldin A blocked by activation of a pertussis-toxin-sensitive G protein.
    Ktistakis et al.
    Nature, 1992;356:344
  3. Isolation of a brefeldin A-inhibited guanine nucleotide-exchange protein for ADP ribosylation factor (ARF)1 and ARF3 that contains a Sec7-like domain.
    Morinaga et al.
    Proc.Natl.Acad.Sci.U.S.A., 1996;93:12856
  4. Differential effects of endoplasmic reticulum stress-induced autophagy on cell survival.
    Ding et al.
    J.Biol.Chem., 2007;282:4702
  5. Identification of a brefeldin A-insensitive guanine nucleotide-exchange protein for ADP-ribosylation factor in bovine brain.
    Tsai et al.
    Proc.Natl.Acad.Sci.U.S.A., 1994;91:3063

The citations listed below are publications that use Tocris products. Selected citations for Brefeldin A include:

Showing Results 1 - 3 of 3

  1. A novel mechanism for nicotinic potentiation of glutamatergic synapses.
    Authors: Halff Et al.
    J Neurosci
  2. The expression and relaxant effect of bitter taste receptors in human bronchi.
    Authors: Grassin-Delyle Et al.
    Respir Res
  3. Concerted actions of NHERF2 and WNK4 in regulating TRPV5.
    Authors: Jing Et al.
    J Neurosci
Tocris Bioscience is the leading supplier of novel and exclusive tools for life science research with over 30 years' experience in the industry. Tocris is a Bio-Techne brand.

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