BRIP1/FANCJ Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-31883
![Western Blot: BRIP1/FANCJ Antibody [NBP1-31883]](https://resources.rndsystems.com/images/products/BRIP1-FANCJ-Antibody-Western-Blot-NBP1-31883-img0017.jpg "Western Blot: BRIP1/FANCJ Antibody [NBP1-31883]")
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Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Proximity Ligation Assay
Cited:
Western Blot, Proximity Ligation Assay
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human BRIP1/FANCJ. The exact sequence is proprietary.
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 26490168).
Localization
Nucleus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
141 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for BRIP1/FANCJ Antibody - BSA Free
Western Blot: BRIP1/FANCJ Antibody [NBP1-31883]
Western Blot: BRIP1/FANCJ Antibody [NBP1-31883] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with FANCJ antibody [N1N2], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.![Immunohistochemistry-Paraffin: BRIP1/FANCJ Antibody [NBP1-31883]](https://resources.rndsystems.com/images/products/BRIP1-FANCJ-Antibody-Immunohistochemistry-Paraffin-NBP1-31883-img0014.jpg "Immunohistochemistry-Paraffin: BRIP1/FANCJ Antibody [NBP1-31883]")
Immunohistochemistry-Paraffin: BRIP1/FANCJ Antibody [NBP1-31883]
Immunohistochemistry-Paraffin: BRIP1/FANCJ Antibody [NBP1-31883] - SAS xenograft. FANCJ antibody [N1N2], N-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunocytochemistry/ Immunofluorescence: BRIP1/FANCJ Antibody [NBP1-31883] -
Immunocytochemistry/ Immunofluorescence: BRIP1/FANCJ Antibody [NBP1-31883] - In situ proximity ligation assay (PLA) in HEK293T cells. (A) G4-BRIP1 & (B) G4-HMOX1 interactions (red dots) were visualized in the control group & after DNase or RNase digestion. Most of the G4 interactions were localized in the nucleus (arrows), but some were also present in the cytoplasm. Cells that were fixed in methanol & mouse 1H6 antibodies were used. Nuclei were counterstained with DAPI (blue), where the transmission bright-field images (BF) show the cell morphologies. Scale bar: 10 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33445471), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Immunocytochemistry/ Immunofluorescence: BRIP1/FANCJ Antibody [NBP1-31883] -
Immunocytochemistry/ Immunofluorescence: BRIP1/FANCJ Antibody [NBP1-31883] - Detection of the PLA signal in sorted hematopoietic stem cells. (A) G4–Brip1 & (B) G4–Hmox1 interactions (green dots) were visualized in HSCs & GMP & MPP cells. G4 interactions were visible in the nucleus (arrows) & in the cytoplasm. Nuclei were counterstained with DAPI (blue), where the transmission bright-field images (BF) show the cell morphologies. Cells were fixed in PFA & goat 1H6 antibodies were used. Scale bars: 5 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33445471), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Western Blot: BRIP1/FANCJ Antibody [NBP1-31883] -
Western Blot: BRIP1/FANCJ Antibody [NBP1-31883] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRIP1/FANCJ antibody [N1N2], N-term (NBP1-31883) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: BRIP1/FANCJ Antibody [NBP1-31883] -
BRIP1/FANCJ antibody [N1N2], N-term detects BRIP1 protein at cytoplasm by immunofluorescent analysis.Sample: 293T cells were fixed in 2% paraformaldehyde/culture medium at 37C for 30 min.
Green: BRIP1 protein stained by BRIP1/FANCJ antibody [N1N2], N-term (NBP1-31883) diluted at 1:500.
Blue: Hoechst 33343 staining.
Applications for BRIP1/FANCJ Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Proximity Ligation Assay
Reported in scientific literature (PMID:33445471)
Western Blot
1:500-1:3000
Application Notes
See Simple Western Antibody Database for Simple Western validation: separated by Size
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Format
BSA Free
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: BRIP1/FANCJ
Long Name
BRCA1 Interacting Protein C-terminal Helicase 1
Alternate Names
BACH1, FACJ, FANCJ
Gene Symbol
BRIP1
Additional BRIP1/FANCJ Products
Product Documents for BRIP1/FANCJ Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for BRIP1/FANCJ Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.Citations for BRIP1/FANCJ Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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