BTN3A3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-88509

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all BTN3A3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SHIYTFPHASFSEPLYPVFRILTLEPTALTICPIPKEVESSPDPDLVPDHSLETPLTPGLANESGEPQAEVTSLLLPAHPGAEVSPSATTNQNHKLQARTEAL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BTN3A3 Antibody - BSA Free

Immunohistochemistry-Paraffin: BTN3A3 Antibody [NBP1-88509]

Immunohistochemistry-Paraffin: BTN3A3 Antibody [NBP1-88509]

Immunohistochemistry-Paraffin: BTN3A3 Antibody [NBP1-88509] - Staining in human spleen and skeletal muscle tissues using NBP1-88509 antibody. Corresponding BTN3A3 RNA-seq data are presented for the same tissues.
Western Blot: BTN3A3 Antibody [NBP1-88509]

Western Blot: BTN3A3 Antibody [NBP1-88509]

Western Blot: BTN3A3 Antibody [NBP1-88509] - Analysis in human cell line Jurkat.
Immunohistochemistry-Paraffin: BTN3A3 Antibody [NBP1-88509]

Immunohistochemistry-Paraffin: BTN3A3 Antibody [NBP1-88509]

Immunohistochemistry-Paraffin: BTN3A3 Antibody [NBP1-88509] - Staining of human fallopian tube shows moderate membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: BTN3A3 Antibody [NBP1-88509]

Immunohistochemistry-Paraffin: BTN3A3 Antibody [NBP1-88509]

Immunohistochemistry-Paraffin: BTN3A3 Antibody [NBP1-88509] - Staining of human lymph node shows strong membranous positivity in non-germinal center cells.
Immunohistochemistry-Paraffin: BTN3A3 Antibody [NBP1-88509]

Immunohistochemistry-Paraffin: BTN3A3 Antibody [NBP1-88509]

Immunohistochemistry-Paraffin: BTN3A3 Antibody [NBP1-88509] - Staining of human rectum shows strong membranous positivity in lymphoid cells.
Immunohistochemistry-Paraffin: BTN3A3 Antibody [NBP1-88509]

Immunohistochemistry-Paraffin: BTN3A3 Antibody [NBP1-88509]

Immunohistochemistry-Paraffin: BTN3A3 Antibody [NBP1-88509] - Staining of human skeletal muscle shows very weak positivity in myocytes as expected.
Immunohistochemistry-Paraffin: BTN3A3 Antibody [NBP1-88509]

Immunohistochemistry-Paraffin: BTN3A3 Antibody [NBP1-88509]

Immunohistochemistry-Paraffin: BTN3A3 Antibody [NBP1-88509] - Staining of human spleen shows strong membranous positivity in cells in red pulp.

Applications for BTN3A3 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BTN3A3

BTN3A3 belongs to the BTN3 subfamily of the butyrophilin (BTN) family. BTN genes are MHC-associated and code for membrane proteins. BTN3A1, BTN3A2 and BTN3A3 exhibit very high homology, but only BTN3A1 and BTN3A3 have an intracellular B30.2 (SPRY) domain in addition to the two extracellular Ig domains. BTN3 (CD277) molecules are found on various immune cells.

Long Name

Butyrophilin, Subfamily 3, Member A3

Alternate Names

BTF3, BTN3.3

Gene Symbol

BTN3A3

Additional BTN3A3 Products

Product Documents for BTN3A3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for BTN3A3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for BTN3A3 Antibody - BSA Free

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Protocols

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