Bub3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-88517

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Bub3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: YQTRCIRAFPNKQGYVLSSIEGRVAVEYLDPSPEVQKKKYAFKCHRLKENNIEQIYPVNAISFHNIHNTFATGGSDGFVNIWDPFNKKRLCQFHRYPTSIASLAFSNDGTTLAIASSYMYEMDDTEHPEDGIFIRQVTDAETKPKSP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit Bub3 Antibody - BSA Free (NBP1-88517) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Bub3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Bub3 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Bub3 Antibody [NBP1-88517]

Immunocytochemistry/ Immunofluorescence: Bub3 Antibody [NBP1-88517]

Immunocytochemistry/Immunofluorescence: Bub3 Antibody [NBP1-88517] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: Bub3 Antibody [NBP1-88517]

Immunohistochemistry-Paraffin: Bub3 Antibody [NBP1-88517]

Immunohistochemistry-Paraffin: Bub3 Antibody [NBP1-88517] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: Bub3 Antibody [NBP1-88517]

Immunohistochemistry-Paraffin: Bub3 Antibody [NBP1-88517]

Immunohistochemistry-Paraffin: Bub3 Antibody [NBP1-88517] - Staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.
Immunohistochemistry-Paraffin: Bub3 Antibody [NBP1-88517]

Immunohistochemistry-Paraffin: Bub3 Antibody [NBP1-88517]

Immunohistochemistry-Paraffin: Bub3 Antibody [NBP1-88517] - Staining of human cervix, uterine shows strong nuclear positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: Bub3 Antibody [NBP1-88517]

Immunohistochemistry-Paraffin: Bub3 Antibody [NBP1-88517]

Immunohistochemistry-Paraffin: Bub3 Antibody [NBP1-88517] - Staining of human Fallopian tube shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: Bub3 Antibody [NBP1-88517]

Immunohistochemistry-Paraffin: Bub3 Antibody [NBP1-88517]

Immunohistochemistry-Paraffin: Bub3 Antibody [NBP1-88517] - Staining of human lymph node shows moderate nuclear positivity in germinal center cells.
Bub3 Antibody - BSA Free Western Blot: Bub3 Antibody - BSA Free [NBP1-88517]

Western Blot: Bub3 Antibody - BSA Free [NBP1-88517]

Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-BUB3 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Bub3 Antibody - BSA Free Western Blot: Bub3 Antibody - BSA Free [NBP1-88517]

Western Blot: Bub3 Antibody - BSA Free [NBP1-88517]

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Applications for Bub3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Bub3

The mitotic checkpoint protein Bub3 appears to be essential during normal mitotic progression, preventing premature sister chromatid separation, missegreation and aneuploidy. Bub3 is also important during G2 and early mitosis stages, promoting normal mitotic entry. Loss of Bub3 function causes slower progression through prophase, apparently due to mitotic cyclins A and B not being accumulated.

Long Name

BUB3 Budding Uninhibited By Benzimidazoles 1 Homolog

Alternate Names

BUB3 (budding uninhibited by benzimidazoles 3, yeast) homolog, BUB3 budding uninhibited by benzimidazoles 3 homolog, BUB3L, budding uninhibited by benomyl, budding uninhibited by benzimidazoles 3 homolog (yeast), hBUB3, mitotic checkpoint component, mitotic checkpoint protein BUB3

Entrez Gene IDs

9184 (Human)

Gene Symbol

BUB3

UniProt

O43684

Additional Bub3 Products

Product Documents for Bub3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Bub3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Bub3 Antibody - BSA Free

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Protocols

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FAQs

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