C. botulinum BoNT-C1 Heavy Chain Receptor Binding Domain Antibody

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Product Details
Supplemental Products

C. botulinum BoNT-C1 Heavy Chain Receptor Binding Domain Antibody Summary

Species Reactivity
C. botulinum
Detects C. botulinum BoNT-C1 Heavy Chain in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross‑reactivity with recombinant (r) BoNT‑D‑HCR is observed and 5% cross-reactivity with rBoNT-A-HCR, rBoNT-A-LC, and rBoNT‑B‑LC is observed.
Polyclonal Sheep IgG
Antigen Affinity-purified
E. coli-derived recombinant BoNT-C1 Heavy Chain
Accession # P18640
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
BoNT-C1 Heavy Chain Receptor Binding Domain
25 µg/mL
Cell lysates spiked with Recombinant C. botulinum BoNT-C1 Heavy Chain, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BoNT-C1 Heavy Chain

BoNT-C1 (Botulinum neurotoxin C1 [heavy chain]) is a 100 kDa C-terminal fragment of the C1 Clostridum botulinum neurotoxin (holo)precursor. It constitutes a nonenzymatic part of a Zn2+-dependent M27 family endoprotease that potently blocks neurosecretion. The inactive 1291 amino acid (aa), 150 kDa C1 precursor is cleaved between Lys449Thr450 to generate a heterodimeric toxin held together by a preexisting interchain disulfide bond between Cys437 and Cys453. The 100 kDa C-terminus (heavy chain) acts as a ligand for neuronal membrane ecto-acceptors, inducing its translocation and internalization. The 50 kDa N-terminal light chain acts as an intracellular peptidase that cleaves SNAP-25 and syntaxin. This is suggested to block noradrenalin release from a readily releasable pool of vesicles. Over aa 866-1291, the C1 heavy chain shares less than 40% aa identity with the D-type neurotoxin heavy chain.                                  

Long Name
Botulinum Neurotoxin Type C1 Heavy Chain
Entrez Gene IDs
10523893 (C. botulinum)
Alternate Names
BoNTC1 Heavy Chain; BoNT-C1 Heavy Chain; DcD

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